Label-free Gram-negative bacteria detection using bacteriophage-adhesin-coated long-period gratings
Ewa Brzozowska
,
Marcin Koba
,
Mateusz Jakub Śmietana
,
Sabina Górska
,
Monika Janik
,
Andrzej Gamian
,
Wojtek J. Bock
Abstract
This paper presents a novel application of a highly sensitive
sensor based on long-period gratings (LPGs) coated with T4 bacteriophage
adhesin for Gram-negative bacteria detection. We show here, that the
sensor evidently recognizes Escherichia coli K-12 (PCM2560), whereas in
the reference tests – ELISA and BIAcore – the results are questionable. For
LPGs sensor the resonant wavelength shift observed for E. coli K-12 was
approximately half of that measured for E.coli B (positive control). The
BIAcore readings (RU) for E. coli K-12 were at 10% level of the signal
obtained for E .coli B. These results confirm the improved sensitivity of the
LPGs sensor. Moreover, we also show that application of adhesin may
allow for efficient detection of E. coli O111 (PCM418), Klebsiella
pneumoniae O1 (PCM1) and Yersinia enterocolitica O1 (PCM1879). The
specificity of binding bacteria by the adhesin is discussed and it is
determined by a distinct region of lipopolysaccharide receptors and/or by
the presence of outer-membrane protein C in an outer membrane of Gram-
negative bacteria.Author |
Ewa Brzozowska - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Ewa Brzozowska,,
-
-
,
Marcin Koba - [Instytut Łączności PIB (IŁ PIB)]
Marcin Koba,,
-
- Instytut Łączności PIB
,
Mateusz Jakub Śmietana (FEIT / MO)
Mateusz Jakub Śmietana,,
- The Institute of Microelectronics and Optoelectronics
,
Sabina Górska - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Sabina Górska,,
-
-
,
Monika Janik - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Monika Janik,,
-
-
,
Andrzej Gamian - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Andrzej Gamian,,
-
-
,
Wojtek J. Bock - [Universite du Quebec en Outaouais]
Wojtek J. Bock,,
-
-
|
Journal series | Biomedical Optics Express, ISSN 2156-7085, (A 40 pkt) |
Issue year | 2016 |
Vol | 7 |
No | 3 |
Pages | 829-840 |
Publication size in sheets | 0.55 |
Keywords in English | Fiber optics sensors; Biology |
ASJC Classification | 3107 Atomic and Molecular Physics, and Optics; 1305 Biotechnology |
DOI | DOI:10.1364/BOE.7.000829 |
URL |
https://www.osapublishing.org/boe/abstract.cfm?uri=boe-7-3-829&origin=search |
Project | x. Project leader: Śmietana Mateusz Jakub,
, Phone: (22) 234 63 64, start date 20-05-2015, planned end date 19-05-2019, IMiO/2015/NCN/2, Implemented
WEiTI
Projects financed by NSC [Projekty finansowane przez NCN]
|
Language | en angielski |
Score (nominal) | 40 |
Score | Ministerial score = 35.0, 06-09-2019, ArticleFromJournal Ministerial score (2013-2016) = 40.0, 06-09-2019, ArticleFromJournal |
Publication indicators |
Scopus Citations = 18;
WoS Citations = 16;
Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.526;
WoS Impact Factor: 2016 = 3.337 (2) - 2016=3.544 (5) |
Citation count* | 19 (2019-12-02) |
Additional fields | |
Acknowledgment | The authors gratefully acknowledge support for this work from the Polish National Science Centre under grants Nos. 2011/03/D/NZ7/02054 and 2014/14/E/ST7/00104, as well as from the Natural Sciences and Engineering Research Council of Canada, and from the Canada Research Chairs Program. |
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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