Label-free Gram-negative bacteria detection using bacteriophage-adhesin-coated long-period gratings

Ewa Brzozowska , Marcin Koba , Mateusz Jakub Śmietana , Sabina Górska , Monika Janik , Andrzej Gamian , Wojtek J. Bock


This paper presents a novel application of a highly sensitive sensor based on long-period gratings (LPGs) coated with T4 bacteriophage adhesin for Gram-negative bacteria detection. We show here, that the sensor evidently recognizes Escherichia coli K-12 (PCM2560), whereas in the reference tests – ELISA and BIAcore – the results are questionable. For LPGs sensor the resonant wavelength shift observed for E. coli K-12 was approximately half of that measured for E.coli B (positive control). The BIAcore readings (RU) for E. coli K-12 were at 10% level of the signal obtained for E .coli B. These results confirm the improved sensitivity of the LPGs sensor. Moreover, we also show that application of adhesin may allow for efficient detection of E. coli O111 (PCM418), Klebsiella pneumoniae O1 (PCM1) and Yersinia enterocolitica O1 (PCM1879). The specificity of binding bacteria by the adhesin is discussed and it is determined by a distinct region of lipopolysaccharide receptors and/or by the presence of outer-membrane protein C in an outer membrane of Gram- negative bacteria.
Author Ewa Brzozowska
Ewa Brzozowska,,
, Marcin Koba - [Instytut Łączności PIB (IŁ PIB)]
Marcin Koba,,
- Instytut Łączności PIB
, Mateusz Jakub Śmietana IMiO
Mateusz Jakub Śmietana,,
- The Institute of Microelectronics and Optoelectronics
, Sabina Górska
Sabina Górska,,
, Monika Janik
Monika Janik,,
, Andrzej Gamian
Andrzej Gamian,,
, Wojtek J. Bock
Wojtek J. Bock,,
Journal seriesBiomedical Optics Express, ISSN 2156-7085
Issue year2016
Publication size in sheets0.55
Keywords in English Fiber optics sensors; Biology
projectx. Project leader: Śmietana Mateusz Jakub, , Phone: (22) 234 63 64, start date 20-05-2015, planned end date 19-05-2019, IMiO/2015/NCN/2, Implemented
WEiTI Projects financed by NSC [Projekty finansowane przez NCN]
Languageen angielski
Score (nominal)40
ScoreMinisterial score [Punktacja MNiSW] = 35.0, 27-03-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 40.0, 27-03-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 3.337 (2) - 2016=3.544 (5)
Citation count*8 (2018-06-18)
Additional fields
AcknowledgmentThe authors gratefully acknowledge support for this work from the Polish National Science Centre under grants Nos. 2011/03/D/NZ7/02054 and 2014/14/E/ST7/00104, as well as from the Natural Sciences and Engineering Research Council of Canada, and from the Canada Research Chairs Program.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.