Label-free Gram-negative bacteria detection using bacteriophage-adhesin-coated long-period gratings

Ewa Brzozowska , Marcin Koba , Mateusz Jakub Śmietana , Sabina Górska , Monika Janik , Andrzej Gamian , Wojtek J. Bock

Abstract

This paper presents a novel application of a highly sensitive sensor based on long-period gratings (LPGs) coated with T4 bacteriophage adhesin for Gram-negative bacteria detection. We show here, that the sensor evidently recognizes Escherichia coli K-12 (PCM2560), whereas in the reference tests – ELISA and BIAcore – the results are questionable. For LPGs sensor the resonant wavelength shift observed for E. coli K-12 was approximately half of that measured for E.coli B (positive control). The BIAcore readings (RU) for E. coli K-12 were at 10% level of the signal obtained for E .coli B. These results confirm the improved sensitivity of the LPGs sensor. Moreover, we also show that application of adhesin may allow for efficient detection of E. coli O111 (PCM418), Klebsiella pneumoniae O1 (PCM1) and Yersinia enterocolitica O1 (PCM1879). The specificity of binding bacteria by the adhesin is discussed and it is determined by a distinct region of lipopolysaccharide receptors and/or by the presence of outer-membrane protein C in an outer membrane of Gram- negative bacteria.
Author Ewa Brzozowska - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Ewa Brzozowska,,
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, Marcin Koba - [Instytut Łączności PIB (IŁ PIB)]
Marcin Koba,,
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- Instytut Łączności PIB
, Mateusz Jakub Śmietana (FEIT / MO)
Mateusz Jakub Śmietana,,
- The Institute of Microelectronics and Optoelectronics
, Sabina Górska - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Sabina Górska,,
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, Monika Janik - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Monika Janik,,
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, Andrzej Gamian - [Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences]
Andrzej Gamian,,
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, Wojtek J. Bock - [Universite du Quebec en Outaouais]
Wojtek J. Bock,,
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Journal seriesBiomedical Optics Express, ISSN 2156-7085, (A 40 pkt)
Issue year2016
Vol7
No3
Pages829-840
Publication size in sheets0.55
Keywords in English Fiber optics sensors; Biology
ASJC Classification3107 Atomic and Molecular Physics, and Optics; 1305 Biotechnology
DOIDOI:10.1364/BOE.7.000829
URL https://www.osapublishing.org/boe/abstract.cfm?uri=boe-7-3-829&origin=search
Projectx. Project leader: Śmietana Mateusz Jakub, , Phone: (22) 234 63 64, start date 20-05-2015, planned end date 19-05-2019, IMiO/2015/NCN/2, Implemented
WEiTI Projects financed by NSC [Projekty finansowane przez NCN]
Languageen angielski
Score (nominal)40
ScoreMinisterial score = 35.0, 06-09-2019, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 06-09-2019, ArticleFromJournal
Publication indicators Scopus Citations = 18; WoS Citations = 16; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.526; WoS Impact Factor: 2016 = 3.337 (2) - 2016=3.544 (5)
Citation count*19 (2019-12-02)
Additional fields
AcknowledgmentThe authors gratefully acknowledge support for this work from the Polish National Science Centre under grants Nos. 2011/03/D/NZ7/02054 and 2014/14/E/ST7/00104, as well as from the Natural Sciences and Engineering Research Council of Canada, and from the Canada Research Chairs Program.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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