An interplay between transcription, processing, and degradation determines tRNA levels in yeast

Dominika Wichtowska , Tomasz W. Turowski , Magdalena Rakowska-Boguta

Abstract

tRNA biogenesis in yeast involves the synthesis of the initial transcript by RNA polymerase III followed by processing and controlled degradation in both the nucleus and the cytoplasm. A vast landscape of regulatory elements controlling tRNA stability in yeast has emerged from recent studies. Diverse pathways of tRNA maturation generate multiple stable and unstable intermediates. A significant impact on tRNA stability is exerted by a variety of nucleotide modifications. Pre-tRNAs are targets of exosome-dependent surveillance in the nucleus. Some tRNAs that are hypomodified or bear specific destabilizing mutations are directed to the rapid tRNA decay pathway leading to 5'→3' exonucleolytic degradation by Rat1 and Xrn1. tRNA molecules are selectively marked for degradation by a double CCA at their 3' ends. In addition, under different stress conditions, tRNA half-molecules can be generated by independent endonucleolytic cleavage events. Recent studies reveal unexpected relationships between the subsequent steps of tRNA biosynthesis and the mechanisms controlling its quality and turnover.
Author Dominika Wichtowska
Dominika Wichtowska,,
-
, Tomasz W. Turowski (FC / IBC / DDTB)
Tomasz W. Turowski,,
- Department Of Drug Technology And Biotechnology
, Magdalena Rakowska-Boguta
Magdalena Rakowska-Boguta,,
-
Journal seriesWiley Interdisciplinary Reviews-RNA, ISSN 1757-7004, [1757-7012]
Issue year2013
Vol4
No6
Pages709-722
Publication size in sheets0.65
ASJC Classification1312 Molecular Biology; 1303 Biochemistry
DOIDOI:10.1002/wrna.1190
URL http://onlinelibrary.wiley.com/doi/10.1002/wrna.1190/abstract
Languageen angielski
File
Wichtowska_et_al-2013-Wiley_Interdisciplinary_Reviews__RNA.pdf 757.59 KB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 30.0, 04-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 04-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 13; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.271; WoS Impact Factor: 2013 = 6.154 (2) - 2013=6.263 (5)
Citation count*5 (2015-11-15)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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