An interplay between transcription, processing, and degradation determines tRNA levels in yeast
Dominika Wichtowska , Tomasz W. Turowski , Magdalena Rakowska-Boguta
AbstracttRNA biogenesis in yeast involves the synthesis of the initial transcript by RNA polymerase III followed by processing and controlled degradation in both the nucleus and the cytoplasm. A vast landscape of regulatory elements controlling tRNA stability in yeast has emerged from recent studies. Diverse pathways of tRNA maturation generate multiple stable and unstable intermediates. A significant impact on tRNA stability is exerted by a variety of nucleotide modifications. Pre-tRNAs are targets of exosome-dependent surveillance in the nucleus. Some tRNAs that are hypomodified or bear specific destabilizing mutations are directed to the rapid tRNA decay pathway leading to 5'→3' exonucleolytic degradation by Rat1 and Xrn1. tRNA molecules are selectively marked for degradation by a double CCA at their 3' ends. In addition, under different stress conditions, tRNA half-molecules can be generated by independent endonucleolytic cleavage events. Recent studies reveal unexpected relationships between the subsequent steps of tRNA biosynthesis and the mechanisms controlling its quality and turnover.
|Journal series||Wiley Interdisciplinary Reviews-RNA, ISSN 1757-7004, [1757-7012]|
|Publication size in sheets||0.65|
|Score|| = 30.0, 04-02-2020, ArticleFromJournal|
= 35.0, 04-02-2020, ArticleFromJournal
|Publication indicators||= 13; : 2013 = 1.271; : 2013 = 6.154 (2) - 2013=6.263 (5)|
|Citation count*||5 (2015-11-15)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.