Projection multiplexing for enhanced acquisition speed in holographic tomography

Arkadiusz Kuś , Maria Baczewska , Michał Ziemczonok , Małgorzata Kujawińska

Abstract

In holographic tomography (HT), the 3D refractive index distribution within weakly-scattering, phase-only biological object is retrieved. This key property of the technique is one of its most significant strengths compared to labelling-based methods of cell analysis such as fluorescence microscopy. As a consequence, however, it is required to acquire a set of holograms at several viewing directions, which hinders the measurement speed. In this paper we explore the prospect of multiplexing projections in order to decrease the number of scanning positions required for the full measurements. The presented analysis is based on experimental data acquired in a limited-angle holographic tomography system and emulates the performance of a spatial-light-modulator-based system in which multiple projections may be acquired simultaneously by generating a distribution of multiple point sources in the Fourier plane of the condenser lens. For this reason, the increase in acquisition speed strictly depends on the number of multiplexed holograms and results in decreased reconstruction quality. The performance of the system is demonstrated and analyzed with biological objects – human keratinocyte cells.
Author Arkadiusz Kuś (FM / IMPh)
Arkadiusz Kuś,,
- The Institute of Micromechanics and Photonics
, Maria Baczewska (FM / IMPh)
Maria Baczewska,,
- The Institute of Micromechanics and Photonics
, Michał Ziemczonok (FM / IMPh)
Michał Ziemczonok,,
- The Institute of Micromechanics and Photonics
, Małgorzata Kujawińska (FM / IMPh)
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics
Pages1088318-1 - 1088318-6
Publication size in sheets0.3
Article number1088318
Book Brown Thomas G., Wilson Tony (eds.): Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVI, Proceedings of SPIE, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10883, 2019, SPIE – The International Society for Optics and Photonics, DOI:10.1117/12.2523423
Keywords in Englishholographic tomography, optical diffraction tomography, tomographic phase microscopy, quantitative phase imaging, 3D refractive index, hologram multiplexing
DOIDOI:10.1117/12.2510628
URL http://spie.org/Publications/Proceedings/Paper/10.1117/12.2510628
projectTEAM TECH BiOpTo: Tomographic phase microscope for biomedical applications. Project leader: Kujawińska Małgorzata, , Phone: +48 22234-8489, start date 01-10-2016, planned end date 31-10-2019, 503/00093, Implemented
WM Program Operacyjny Inteligentny Rozwój (PO IR)
Languageen angielski
Score (nominal)15
ScoreMinisterial score = 15.0, 11-07-2019, ChapterFromConference
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