A novel hybrid nanofibrous strategy to target progenitor cells for a cost-effective in situ angiogenesis

Nadine Sachot , Oscar Castano , Hugo Oliverira , Joan Marti-Munoz , Agata Roguska , Joel Amedee , Małgorzata Lewandowska , Joseph A. Planell , Elisabeth Engel

Abstract

Although the impact of composites based on Ti-doped calcium phosphate glasses is low compared with that of bioglass, they have been already shown to possess great potential for bone tissue engineering. Composites made of polylactic acid (PLA) and a microparticle glass of 5TiO2–44.5CaO–44.5P2O5–6Na2O (G5) molar ratio have already demonstrated in situ osteo- and angiogenesis-triggering abilities. As many of the hybrid materials currently developed usually promote osteogenesis but still lack the ability to induce vascularization, a G5/PLA combination is a cost-effective option for obtaining new instructive scaffolds. In this study, nanostructured PLA-ORMOGLASS (organically modified glass) fibers were produced by electrospinning, in order to fabricate extra-cellular matrix (ECM)-like substrates that simultaneously promote bone formation and vascularization. Physical–chemical and surface characterization and tensile tests demonstrated that the obtained scaffolds exhibited homogeneous morphology, higher hydrophilicity and enhanced mechanical properties than pure PLA. In vitro assays with rat mesenchymal stem cells (rMSCs) and rat endothelial progenitor cells (rEPCs) also showed that rMSCs attached and proliferated on the materials influenced by the calcium content in the environment. In vivo assays showed that hybrid composite PLA-ORMOGLASS fibers were able to promote the formation of blood vessels. Thus, these novel fibers are a valid option for the design of functional materials for tissue engineering applications.
Author Nadine Sachot - Institute for Bioengineering of Catalonia
Nadine Sachot,,
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, Oscar Castano - Institute for Bioengineering of Catalonia
Oscar Castano,,
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, Hugo Oliverira - University of Bordeaux
Hugo Oliverira,,
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, Joan Marti-Munoz - Institute for Bioengineering of Catalonia
Joan Marti-Munoz,,
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, Agata Roguska - [Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN)]
Agata Roguska,,
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- Instytut Chemii Fizycznej Polskiej Akademii Nauk
, Joel Amedee - University of Bordeaux
Joel Amedee,,
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, Małgorzata Lewandowska ZPM
Małgorzata Lewandowska,,
- Division of Materials Design
, Joseph A. Planell - Institute for Bioengineering of Catalonia
Joseph A. Planell,,
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, Elisabeth Engel - Institute for Bioengineering of Catalonia
Elisabeth Engel,,
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Journal seriesJournal of Materials Chemistry B: materials for biology and medicine, ISSN 2050-750X
Issue year2016
Vol4
Pages6967-6978
Publication size in sheets0.55
DOIDOI:10.1039/c6tb02162j
Languageen angielski
File
2016 J Mater Chem B.pdf 5.49 MB
Score (nominal)35
ScoreMinisterial score [Punktacja MNiSW] = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 4.543 (2) - 2016=4.787 (5)
Citation count*0
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