In vitro and in vivo genotoxicity assessment of selected pharmaceuticals in relation to Escherichia coli and Cyprinus carpio
Monika Załęska-Radziwiłł , Katarzyna Affek , Nina Doskocz , A. Affek
AbstractGenotoxicity studies (using SOS chromotest and comet assay) of Escherichia coli and carp (Cyprinus carpio) were performed for three pharmaceutically active compounds, ciprofloxacin, 17α-ethinylestradiol and 5-fluorouracil, used in the treatment of humans. The values of genotoxicity induction coefficient (I) in the SOS chromotest clearly showed genotoxicity for ciprofloxacin, both in the presence and in the absence of S9 fraction; 17α-ethinylestradiol demonstrated slight genotoxicity at the highest tested concentration; and 5-fluorouracil did not induce genotoxic effects in Escherichia coli mutants. Statistical analysis of the results of the comet assay revealed significant differences in cell populations derived from carp placed in a solution of 5-fluorouracil in comparison with the negative control. Statistical analysis also showed a significant increase of “% DNA in tail” of comets in cell populations incubated in solutions of 17α-ethinylestradiol at concentrations of 10000, 2000 and 400 µg/L and in solutions of 5-fluorouracil with S9 fraction at concentrations of 50,000 and 2,000 μg/L in comparison with the negative controls.
|Journal series||Journal of Environmental Science and Health Part Atoxic/hazardous Substances & Environmental Engineering, ISSN 1093-4529|
|Publication size in sheets||0.5|
|Keywords in English||5-fluorouracil, 17α-ethinylestradiol, ciprofloxacin, environmental genotoxicity, SOS chromotest, comet assay|
|Score|| = 20.0, 28-11-2017, ArticleFromJournal|
= 20.0, 28-11-2017, ArticleFromJournal
|Publication indicators||: 2016 = 1.425 (2) - 2016=1.455 (5)|
|Citation count*||2 (2018-06-14)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.