In vitro and in vivo genotoxicity assessment of selected pharmaceuticals in relation to Escherichia coli and Cyprinus carpio

Monika Załęska-Radziwiłł , Katarzyna Affek , Nina Doskocz , A. Affek

Abstract

Genotoxicity studies (using SOS chromotest and comet assay) of Escherichia coli and carp (Cyprinus carpio) were performed for three pharmaceutically active compounds, ciprofloxacin, 17α-ethinylestradiol and 5-fluorouracil, used in the treatment of humans. The values of genotoxicity induction coefficient (I) in the SOS chromotest clearly showed genotoxicity for ciprofloxacin, both in the presence and in the absence of S9 fraction; 17α-ethinylestradiol demonstrated slight genotoxicity at the highest tested concentration; and 5-fluorouracil did not induce genotoxic effects in Escherichia coli mutants. Statistical analysis of the results of the comet assay revealed significant differences in cell populations derived from carp placed in a solution of 5-fluorouracil in comparison with the negative control. Statistical analysis also showed a significant increase of “% DNA in tail” of comets in cell populations incubated in solutions of 17α-ethinylestradiol at concentrations of 10000, 2000 and 400 µg/L and in solutions of 5-fluorouracil with S9 fraction at concentrations of 50,000 and 2,000 μg/L in comparison with the negative controls.
Author Monika Załęska-Radziwiłł ZB
Monika Załęska-Radziwiłł,,
- Department of Biology
, Katarzyna Affek ZB
Katarzyna Affek,,
- Department of Biology
, Nina Doskocz ZB
Nina Doskocz,,
- Department of Biology
, A. Affek
A. Affek,,
-
Journal seriesJournal of Environmental Science and Health Part Atoxic/hazardous Substances & Environmental Engineering, ISSN 1093-4529
Issue year2016
Vol51
No12
Pages1053-1061
Publication size in sheets0.5
Keywords in English5-fluorouracil, 17α-ethinylestradiol, ciprofloxacin, environmental genotoxicity, SOS chromotest, comet assay
DOIDOI:10.1080/10934529.2016.1198625
Languageen angielski
Score (nominal)20
ScoreMinisterial score [Punktacja MNiSW] = 20.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 20.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 1.425 (2) - 2016=1.455 (5)
Citation count*2 (2018-06-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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