Disposable, acetylcholinesterase-coated, screen-printed carbon electrodes for the determination of organophosphorus pesticides

Piotr Walter , Andrzej Pepłowski , Łukasz Górski , Daniel Janczak , Małgorzata Jakubowska

Abstract

Because of the bioaccumulation effect, organophosphorus pesticides cause long-term damage to mammals, even at small concentrations. The ability to perturb the phospholipid bilayer structure as well as the overstimulation of cholinergic receptors makes them hazardous to humans. Therefore, there is a need for a quick and inexpensive detection of organophosphorus pesticides for agricultural and household use. As organophosphorus pesticides are acetylcholinesterase (AChE) inhibitors, biosensors using this mechanism hold a great promise to meet these requirements with a fraction of reagents and time used for measurement comparing to laboratory methods. This study aims to manufacture AChE-coated, screen-printed carbon electrodes applicable in such amperometric biosensors. AChE enzyme, known for catalytic activity for the hydrolysis of acetylthiocholine (ATCh), could be used to obtain electrochemically active thiocholine from acetylthiocholine chloride in aqueous solutions. Using Malathion’s inhibitory effect towards AChE, pesticides’ presence can be detected by reduction of anodic oxidation peaks of thiocholine in cyclic voltammetry. The conducted research proved that it is possible to detect pesticides using low-cost, simple-to-manufacture screen-printed graphite (GR) electrodes with an enzymatic (AChE) coating. Investigated electrodes displayed significant catalytic activity to the hydrolysis of ATCh. Owing to inhibition effect of the enzyme, amperometric response of the samples decreased in pesticide-spiked solution, allowing determination of organophosphorus pesticides. Printed electronics has grown significantly in recent years as well as research focused on carbon-based nanocomposites. Yet, the utilization of carbon nanocomposites in screen-printed electronics is still considered a novelty in the market. Biosensors have proved useful not only in laboratory conditions but also in home applications, as glucometers are a superior solution for glucose determination for personal use. Although pesticides could be detected accurately using chromatography, spectroscopy, spectrometry or spectrophotometry, the market lacks low-cost, disposable solutions for pesticide detection applicable for household use. With biosensing techniques and electric paths screen-printed with GR or graphene nanocomposites, this preliminary research focuses on meeting these needs.
Author Piotr Walter (FM / IMBE)
Piotr Walter,,
- The Institute of Metrology and Biomedical Engineering
, Andrzej Pepłowski (FM / IMBE)
Andrzej Pepłowski,,
- The Institute of Metrology and Biomedical Engineering
, Łukasz Górski (FC / CMB)
Łukasz Górski,,
- Chair of Medical Biotechnology
, Daniel Janczak (FM / IMBE)
Daniel Janczak,,
- The Institute of Metrology and Biomedical Engineering
, Małgorzata Jakubowska (FM / IMBE)
Małgorzata Jakubowska,,
- The Institute of Metrology and Biomedical Engineering
Journal seriesMicroelectronics International, ISSN 1356-5362, e-ISSN 1758-812X, (N/A 40 pkt)
Issue year2019
Vol36
No3
Pages120-126
Publication size in sheets0.5
Keywords in Englishbiosensor, electrode, acetylcholinesterase, pesticides, screen-printed electronics, screen-printing, malathion, enzymatic electrodes, organophosphorus pesticides
ASJC Classification2208 Electrical and Electronic Engineering; 2504 Electronic, Optical and Magnetic Materials; 2508 Surfaces, Coatings and Films; 3104 Condensed Matter Physics; 3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.1108/MI-12-2018-0084
URL https://www.emerald.com/insight/content/doi/10.1108/MI-12-2018-0084/full/html
Languageen angielski
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wdpb_publikacje_pliki_plik_publikacja_3690_org.pdf 1.88 MB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 07-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.487; WoS Impact Factor: 2018 = 0.84 (2) - 2018=0.727 (5)
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