Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration

Nehar Celikkin , Simone Mastrogiacomo , Jakub Jaroszewicz , X.F. Walboomers , Wojciech Święszkowski

Abstract

Gelatin methacrylate (GelMA) is an inexpensive, photocrosslinkable, cell-responsive hydrogel which has drawn attention for a wide range of tissue engineering appli- cations. The potential of GelMA scaffolds was demonstrated to be tunable for different tissue engineering (TE) applica- tions through modifying the polymer concentration, metha- crylation degree, or UV light intensity. Despite the promising results of GelMA hydrogels in tissue engineering, the influ- ence of polymer concentration for bone tissue engineering (BTE) scaffolds was not established yet. Thus, in this study, we have demonstrated the effect of polymer concentration in GelMA scaffolds on osteogenic differentiation. We prepared GelMA scaffolds with 5 and 10% polymer concentrations and characterized the scaffolds in terms of porosity, pore size, swelling characteristics, and mechanical properties. Subse- quent to the scaffolds characterization, the scaffolds were seeded with bone marrow derived rat mesenchymal stem cells and cultured in osteogenic media to evaluate the possi- ble osteogenic differentiation effect exerted by the polymer concentration. After 7, 14, 21, and 28 days, DNA content, cal- cium deposition, and alkaline phosphatase (ALP) activity of scaffolds were evaluated quantitatively by colorimetric bioas- says. Furthermore, the distribution of the calcium deposition within the scaffolds was attained qualitatively and quantitatively by microcomputer tomography ( m CT). Our data suggest that GelMA hydrogels prepared with 5% polymer concentration has promoted homogeneous extracellular matrix calcification and it is a great candidate for BTE applications
Author Nehar Celikkin (FMSE / DMD)
Nehar Celikkin,,
- Division of Materials Design
, Simone Mastrogiacomo - [Radboud University Nijmegen Medical Centre]
Simone Mastrogiacomo,,
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, Jakub Jaroszewicz (FMSE)
Jakub Jaroszewicz,,
- Faculty of Materials Science and Engineering
, X.F. Walboomers - [Radboud University Nijmegen Medical Centre]
X.F. Walboomers,,
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, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
Journal seriesJournal of Biomedical Materials Research Part A, ISSN 1549-3296, [1552-4965], (A 35 pkt)
Issue year2018
Vol106
No1
Pages201-209
Publication size in sheets0.5
Keywords in EnglishGelMA hydrogels, bone tissue engineering, effect of polymer concentration, m CT imaging of hydrogels
ASJC Classification2506 Metals and Alloys; 2204 Biomedical Engineering; 2502 Biomaterials; 2503 Ceramics and Composites
DOIDOI:10.1002/jbm.a.36226
URL https://onlinelibrary.wiley.com/doi/abs/10.1002/jbm.a.36226
Languageen angielski
File
10.1002jbm.a.36226.pdf 735.41 KB
Score (nominal)40
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.054; WoS Impact Factor: 2017 = 3.231 (2) - 2017=3.329 (5); Scopus Citations = 6; WoS Citations = 7
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