3D bioprinted hydrogel model incorporatingβ-tricalcium phosphate for calcified cartilage tissue engineering

Alicja Kosik-Kozioł , Marco Costantini , Anna Mróz , Joanna Idaszek , Marcin Heljak , Jakub Jaroszewicz , Ewa Kijeńska-Gawrońska , Krisztina Szöke , Nadine Frerker , Andrea Barbetta , Jan Brinchmann , Wojciech Święszkowski


One promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while - despite its key role in the osteochondral region - only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.
Author Alicja Kosik-Kozioł (FMSE / DMD)
Alicja Kosik-Kozioł,,
- Division of Materials Design
, Marco Costantini - Università Campus Bio-Medico di Roma [Warsaw University of Technology (PW)]
Marco Costantini,,
- Politechnika Warszawska
, Anna Mróz - [Politechnika Warszawska]
Anna Mróz,,
, Joanna Idaszek (FMSE / DMD)
Joanna Idaszek,,
- Division of Materials Design
, Marcin Heljak (FMSE / DMD)
Marcin Heljak,,
- Division of Materials Design
, Jakub Jaroszewicz (FMSE / DMD)
Jakub Jaroszewicz,,
- Division of Materials Design
, Ewa Kijeńska-Gawrońska (FMSE / DMD)
Ewa Kijeńska-Gawrońska,,
- Division of Materials Design
, Krisztina Szöke - [Oslo University Hospital]
Krisztina Szöke,,
, Nadine Frerker - [Oslo University Hospital]
Nadine Frerker,,
, Andrea Barbetta - Sapienza University of Rome [Universita degli Studi di Roma La Sapienza]
Andrea Barbetta,,
et al.`
Journal seriesBiofabrication, ISSN 1758-5082, e-ISSN 1758-5090
Issue year2019
Publication size in sheets0.6
Article number035016
Keywords in Englishalginate, gelatin methacrylate, β-tricalcium phosphate TCP, bioprinting, coaxial needle, calcified cartilage
ASJC Classification2700 General Medicine; 1303 Biochemistry; 1305 Biotechnology; 1502 Bioengineering; 2204 Biomedical Engineering; 2502 Biomaterials
URL https://iopscience.iop.org/article/10.1088/1758-5090/ab15cb/pdf
Languageen angielski
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 17-09-2020, ArticleFromJournal
Publication indicators Scopus Citations = 18; GS Citations = 17.0; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.236; WoS Impact Factor: 2018 = 7.236 (2) - 2018=7.35 (5)
Citation count*17 (2020-09-05)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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