3D bioprinted hydrogel model incorporatingβ-tricalcium phosphate for calcified cartilage tissue engineering
Alicja Kosik-Kozioł , Marco Costantini , Anna Mróz , Joanna Idaszek , Marcin Heljak , Jakub Jaroszewicz , Ewa Kijeńska-Gawrońska , Krisztina Szöke , Nadine Frerker , Andrea Barbetta , Jan Brinchmann , Wojciech Święszkowski
AbstractOne promising strategy to reconstruct osteochondral defects relies on 3D bioprinted three-zonal structures comprised of hyaline cartilage, calcified cartilage, and subchondral bone. So far, several studies have pursued the regeneration of either hyaline cartilage or bone in vitro while - despite its key role in the osteochondral region - only few of them have targeted the calcified layer. In this work, we present a 3D biomimetic hydrogel scaffold containing β-tricalcium phosphate (TCP) for engineering calcified cartilage through a co-axial needle system implemented in extrusion-based bioprinting process. After a thorough bioink optimization, we showed that 0.5% w/v TCP is the optimal concentration forming stable scaffolds with high shape fidelity and endowed with biological properties relevant for the development of calcified cartilage. In particular, we investigate the effect induced by ceramic nano-particles over the differentiation capacity of bioprinted bone marrow-derived human mesenchymal stem cells in hydrogel scaffolds cultured up to 21 d in chondrogenic media. To confirm the potential of the presented approach to generate a functional in vitro model of calcified cartilage tissue, we evaluated quantitatively gene expression of relevant chondrogenic (COL1, COL2, COL10A1, ACAN) and osteogenic (ALPL, BGLAP) gene markers by means of RT-qPCR and qualitatively by means of fluorescence immunocytochemistry.
|Journal series||Biofabrication, ISSN 1758-5082, e-ISSN 1758-5090, (N/A 140 pkt)|
|Publication size in sheets||0.6|
|Keywords in English||alginate, gelatin methacrylate, β-tricalcium phosphate TCP, bioprinting, coaxial needle, calcified cartilage|
|ASJC Classification||; ; ; ; ;|
|Score||= 140.0, 10-01-2020, ArticleFromJournal|
|Publication indicators||= 3; = 5.0; = 3; : 2018 = 1.236; : 2018 = 7.236 (2) - 2018=7.35 (5)|
|Citation count*||6 (2020-01-21)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.