Bacteriophage Adhesin-Coated Long-Period Grating-Based Sensor: Bacteria Detection Specificity

Marcin Koba , Mateusz Jakub Śmietana , Ewa Brzozowska , Sabina Górska , Monika Janik , Predrag Mikulic , Andrea Cusano , Wojtek J. Bock

Abstract

In this paper, we study a label-free detection specificity of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point of higher order cladding modes. The LPG sensor is functionalized with bacteriophage adhesin and tested with specific and nonspecific bacteria dry weights. It is shown that that such a biosensor is able to selectively bind to specific bacterial strains. In the experiments bacteria dry weights of E. coli B were used as a positive test and E. coli K12 and Salmonella enterica as negative tests. The resonance wavelength shift induced by E. coli B reaches over 90 nm, while for E. coli K12 and Salmonella enterica approximately 40 and 20 nm, respectively. Additionally, whole-cell-ELISA tests were made to confirm LPG-based biosensor outcomes, and numerical simulations were conducted to estimate biolayer impact on sensor's performance.
Author Marcin Koba - [Instytut Łączności PIB (IŁ PIB)]
Marcin Koba,,
-
- Instytut Łączności PIB
, Mateusz Jakub Śmietana IMiO
Mateusz Jakub Śmietana,,
- The Institute of Microelectronics and Optoelectronics
, Ewa Brzozowska
Ewa Brzozowska,,
-
, Sabina Górska
Sabina Górska,,
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, Monika Janik
Monika Janik,,
-
, Predrag Mikulic
Predrag Mikulic,,
-
, Andrea Cusano
Andrea Cusano,,
-
, Wojtek J. Bock
Wojtek J. Bock,,
-
Journal seriesJournal of Lightwave Technology, ISSN 0733-8724
Issue year2016
Vol34
No19
Pages4531 - 4536
Publication size in sheets0.5
Keywords in EnglishBacteria detection, bacteriophage adhesin, biosensors, label-free sensing, long-period gratings, optical fiber sensors, surface functionalization.
DOIDOI:10.1109/JLT.2016.2532466
URL http://ieeexplore.ieee.org/document/7414382
projectThe Development of Design, Processing and Testing Methods of the Electronic Devices and Materials for Microelectronics and Optoelectronics. Project leader: Szczepański Paweł, , Phone: (48 22) 234 58 70, start date 01-01-2015, planned end date 31-12-2015, end date 31-05-2016, IMiO/2015/STATUT/1, Implemented
WEiTI Działalność statutowa
Languageen angielski
Score (nominal)35
ScoreMinisterial score = 35.0, 27-03-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 27-03-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 3.671 (2) - 2016=3.35 (5)
Citation count*8 (2018-01-22)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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