Bacteriophage Adhesin-Coated Long-Period Grating-Based Sensor: Bacteria Detection Specificity
Marcin Koba , Mateusz Jakub Śmietana , Ewa Brzozowska , Sabina Górska , Monika Janik , Predrag Mikulic , Andrea Cusano , Wojtek J. Bock
AbstractIn this paper, we study a label-free detection specificity of an optical fiber long-period grating (LPG) biosensor working near the dispersion turning point of higher order cladding modes. The LPG sensor is functionalized with bacteriophage adhesin and tested with specific and nonspecific bacteria dry weights. It is shown that that such a biosensor is able to selectively bind to specific bacterial strains. In the experiments bacteria dry weights of E. coli B were used as a positive test and E. coli K12 and Salmonella enterica as negative tests. The resonance wavelength shift induced by E. coli B reaches over 90 nm, while for E. coli K12 and Salmonella enterica approximately 40 and 20 nm, respectively. Additionally, whole-cell-ELISA tests were made to confirm LPG-based biosensor outcomes, and numerical simulations were conducted to estimate biolayer impact on sensor's performance.
|Journal series||Journal of Lightwave Technology, ISSN 0733-8724|
|Pages||4531 - 4536|
|Publication size in sheets||0.5|
|Keywords in English||Bacteria detection, bacteriophage adhesin, biosensors, label-free sensing, long-period gratings, optical fiber sensors, surface functionalization.|
|project||The Development of Design, Processing and Testing Methods of the Electronic Devices and Materials for Microelectronics and Optoelectronics. Project leader: Szczepański Paweł,
, Phone: (48 22) 234 58 70, start date 01-01-2015, planned end date 31-12-2015, end date 31-05-2016, IMiO/2015/STATUT/1, Implemented
|Score|| = 35.0, 27-03-2017, ArticleFromJournal|
= 35.0, 27-03-2017, ArticleFromJournal
|Publication indicators||: 2016 = 3.671 (2) - 2016=3.35 (5)|
|Citation count*||8 (2018-01-22)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.