Identification of the tri-Al tricitrate complex in Plantago almogravensis by hydrophilic interaction LC with parallel ICP-MS and electrospray Orbitrap MS/MS detection

Tomas Grevenstuk , Paulina Flis , L. Ouerdane , Ryszard Łobiński , A. Romano


The identification of the ligands binding Al is essential to understand the mechanisms by which plants detoxify Al internally. However, studies concerning the speciation of Al have been frustrated by its complex chemistry. This work describes the identification of the tri-Al tricitrate (Al3cit3) complex in Plantago almogravensis, encompassing an integrated mass spectrometry approach based on hydrophilic interaction liquid chromatography (HILIC) and parallel detection by ICP-MS and ESI-MS/MS. This work also reports that both Al and Fe are bound by tricitrate, sometimes simultaneously, and the consequences of this finding are discussed. Of the complexes separated by size exclusion chromatography, Al3cit3 is the most stable occurring in P. almogravensis as it was the only one recovered after HILIC. This approach provided new information on the mechanism of Al detoxification in P. almogravensis, namely that Al is bound by the organic acid citrate and that the relative concentration of the detected complexes is affected by the organ type and internal Al concentration, and has potential for studying the speciation of Al in less tolerant plants.
Author Tomas Grevenstuk - [IBB]
Tomas Grevenstuk,,
, Paulina Flis - [Universite de Pau et des Pays de L'Adour]
Paulina Flis,,
, L. Ouerdane - [Universite de Pau et des Pays de L'Adour]
L. Ouerdane,,
, Ryszard Łobiński (FC / CAC)
Ryszard Łobiński,,
- Chair Of Analytical Chemistry
, A. Romano - [IBB]
A. Romano,,
Journal seriesMetallomics, ISSN 1756-5901
Issue year2013
Publication size in sheets0.5
ASJC Classification2506 Metals and Alloys; 1303 Biochemistry; 2502 Biomaterials; 1304 Biophysics; 1601 Chemistry (miscellaneous)
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_1969_org.pdf 1.93 MB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 01-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 01-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 8; WoS Citations = 7; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.09; WoS Impact Factor: 2013 = 3.978 (2) - 2013=4.01 (5)
Citation count*1 (2015-02-27)
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