Evaluation of biological activity of quantum dots in a microsystem

Ilona Grabowska-Jadach , Maja Haczyk , Marcin Drozd , Agnieszka Fischer , Mariusz Pietrzak , Elżbieta Malinowska , Zbigniew Brzózka

Abstract

The presented work aimed at systematic investigation of biological activity of CdSexS1−x/ZnS and CdSe/ZnS quantum dots (QDs), whose surface was modified with different ligands. For these studies, we used a microfluidic system combined with fluorescence microscopy techniques, which enabled analysis of cells' morphology, viability, and QDs uptake. PDMS and glass-based microfluidic system enabled the precise control of the cell environment, allowed to examine five replications of each tested QDs concentrations (statistically significant number), monitor multiple cellular events, and avoid manual preparation of QDs dilutions. We investigated the influence of the core composition and the type of surface modifiers on QDs toxicity. We also determined whether the examined nanoparticles penetrate into the cells. For all tested nanoparticles, the decrease of cells' viability was observed when increasing nanoparticles concentration. The decrease of live cells' number in microchambers and the accumulation of the nanoparticles around cultured cells were observed. The effect of hydrocarbon chain length of surface modifiers and QDs core composition on the cell viability was confirmed in our tests.
Author Ilona Grabowska-Jadach ZMB
Ilona Grabowska-Jadach,,
- Department Of Microbioanalytics
, Maja Haczyk ZMB
Maja Haczyk,,
- Department Of Microbioanalytics
, Marcin Drozd ZMB
Marcin Drozd,,
- Department Of Microbioanalytics
, Agnieszka Fischer ZMB
Agnieszka Fischer,,
- Department Of Microbioanalytics
, Mariusz Pietrzak ZMB
Mariusz Pietrzak,,
- Department Of Microbioanalytics
, Elżbieta Malinowska ZMB
Elżbieta Malinowska,,
- Department Of Microbioanalytics
, Zbigniew Brzózka ZMB
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesElectrophoresis, ISSN 0173-0835
Issue year2016
Vol37
No3, sp. issue
Pages425-431
Publication size in sheets0.5
Keywords in EnglishCell culture; Cells’ viability; Cytotoxicity; Microfluidic system; Quantum dots
DOIDOI:10.1002/elps.201500294
URL http://onlinelibrary.wiley.com/doi/10.1002/elps.201500294/abstract
Languageen angielski
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Grabowska-Jadach_et_al-2016-ELECTROPHORESIS.pdf (file archived - login or check accessibility on faculty) Grabowska-Jadach_et_al-2016-ELECTROPHORESIS.pdf 651.07 KB
Score (nominal)30
ScoreMinisterial score = 25.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.744 (2) - 2016=2.487 (5)
Citation count*2 (2016-09-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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