Evaluation of biological activity of quantum dots in a microsystem

Ilona Grabowska-Jadach , Maja Haczyk , Marcin Drozd , Agnieszka Fischer , Mariusz Pietrzak , Elżbieta Malinowska , Zbigniew Brzózka


The presented work aimed at systematic investigation of biological activity of CdSexS1−x/ZnS and CdSe/ZnS quantum dots (QDs), whose surface was modified with different ligands. For these studies, we used a microfluidic system combined with fluorescence microscopy techniques, which enabled analysis of cells' morphology, viability, and QDs uptake. PDMS and glass-based microfluidic system enabled the precise control of the cell environment, allowed to examine five replications of each tested QDs concentrations (statistically significant number), monitor multiple cellular events, and avoid manual preparation of QDs dilutions. We investigated the influence of the core composition and the type of surface modifiers on QDs toxicity. We also determined whether the examined nanoparticles penetrate into the cells. For all tested nanoparticles, the decrease of cells' viability was observed when increasing nanoparticles concentration. The decrease of live cells' number in microchambers and the accumulation of the nanoparticles around cultured cells were observed. The effect of hydrocarbon chain length of surface modifiers and QDs core composition on the cell viability was confirmed in our tests.
Author Ilona Grabowska-Jadach (FC / IBC / DM)
Ilona Grabowska-Jadach,,
- Department Of Microbioanalytics
, Maja Haczyk (FC / IBC / DM)
Maja Haczyk,,
- Department Of Microbioanalytics
, Marcin Drozd (FC / IBC / DM)
Marcin Drozd,,
- Department Of Microbioanalytics
, Agnieszka Fischer (FC / IBC / DM)
Agnieszka Fischer,,
- Department Of Microbioanalytics
, Mariusz Pietrzak (FC / IBC / DM)
Mariusz Pietrzak,,
- Department Of Microbioanalytics
, Elżbieta Malinowska (FC / IBC / DM)
Elżbieta Malinowska,,
- Department Of Microbioanalytics
, Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesElectrophoresis, ISSN 0173-0835
Issue year2016
No3, sp. issue
Publication size in sheets0.5
Keywords in EnglishCell culture; Cells’ viability; Cytotoxicity; Microfluidic system; Quantum dots
ASJC Classification1308 Clinical Biochemistry; 1303 Biochemistry
URL http://onlinelibrary.wiley.com/doi/10.1002/elps.201500294/abstract
Languageen angielski
Grabowska-Jadach_et_al-2016-ELECTROPHORESIS.pdf 651.07 KB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 25.0, 17-08-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 17-08-2020, ArticleFromJournal
Publication indicators WoS Citations = 4; Scopus Citations = 6; GS Citations = 2.0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.773; WoS Impact Factor: 2016 = 2.744 (2) - 2016=2.487 (5)
Citation count*2 (2016-09-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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