Surface endothelialization of polyurethanes
Beata Aleksandra Butruk , Piotr Bąbik , Barbara Marczak , Tomasz Ciach
AbstractObjectives: The challenge for cardiovascular tissue engineers is to design hemocompatible biomaterials that promote neo-tissue formation. Despite many years of intensive research materials used to fabricate synthetic implants still cause formation of clots in the bloodstream, which can have fatal consequences for the patient. The most promising strategy to fabricate non-thrombogenic materials is covering the implant surface with a layer of endothelial cells. Methods & Materials: In the presented work, a method to modify polyurethane surface is proposed. The surface-modified materials are designed to selectively promote endothelial cell adhesion and proliferation. The modification is based on three-step process: surface activation, introduction of spacer and coupling peptide to spacer molecule. To determine the presence of surface functional groups after each step of the process, colorimetric assays were applied. As a modifying peptide we decided to use peptides containing REDV sequence, which has been proved to promote ECs adhesion and proliferation. In order to confirm the successful incorporation of peptides and visualize their surface distribution a set of materials with fluorescence stained peptides was prepared and analyzed with fluorescence microscopy. Results: Results of HUVEC seeding onto modified materials showed that introduced peptide layer promotes endo-thelial cells adhesion. The majority of cells cultured with modified polymer were alive, with proper morphology. Moreover, during 24-hour period of incubation cells adhered to REDV-modified surface started to proliferate. Conclusions: Surface endothelialization of medical polymers will result in developing of highly hemocompatible hybrid implant - polymeric structure coated with patient’s living cells.
|Journal series||International Journal of Artificial Organs, ISSN 0391-3988|
|Publication size in sheets||0.3|
|Conference||XL Congress of the European Society for Artificial Organs (Esao2013), 11-09-2013 - 14-09-2013, Glasgow, Wielka Brytania|
|Keywords in English||blood coagulation, endothelial cell, endothelialization, hemocompatibility, polyurethane, REDV peptide, surface modification|
|ASJC Classification||; ; ; ;|
|Score|| = 20.0, 07-04-2020, ArticleFromJournalAndMatConfByConferenceseries|
= 20.0, 07-04-2020, ArticleFromJournalAndMatConfByConferenceseries
|Publication indicators||= 5; = 8.0; : 2013 = 0.599; : 2013 = 1.448 (2) - 2013=1.454 (5)|
|Citation count*||8 (2020-01-13)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.