Surface endothelialization of polyurethanes

Beata Aleksandra Butruk , Piotr Bąbik , Barbara Marczak , Tomasz Ciach

Abstract

Objectives: The challenge for cardiovascular tissue engineers is to design hemocompatible biomaterials that promote neo-tissue formation. Despite many years of intensive research materials used to fabricate synthetic implants still cause formation of clots in the bloodstream, which can have fatal consequences for the patient. The most promising strategy to fabricate non-thrombogenic materials is covering the implant surface with a layer of endothelial cells. Methods & Materials: In the presented work, a method to modify polyurethane surface is proposed. The surface-modified materials are designed to selectively promote endothelial cell adhesion and proliferation. The modification is based on three-step process: surface activation, introduction of spacer and coupling peptide to spacer molecule. To determine the presence of surface functional groups after each step of the process, colorimetric assays were applied. As a modifying peptide we decided to use peptides containing REDV sequence, which has been proved to promote ECs adhesion and proliferation. In order to confirm the successful incorporation of peptides and visualize their surface distribution a set of materials with fluorescence stained peptides was prepared and analyzed with fluorescence microscopy. Results: Results of HUVEC seeding onto modified materials showed that introduced peptide layer promotes endo-thelial cells adhesion. The majority of cells cultured with modified polymer were alive, with proper morphology. Moreover, during 24-hour period of incubation cells adhered to REDV-modified surface started to proliferate. Conclusions: Surface endothelialization of medical polymers will result in developing of highly hemocompatible hybrid implant - polymeric structure coated with patient’s living cells.
Author Beata Aleksandra Butruk (FCPE / DBBE)
Beata Aleksandra Butruk,,
- Department of Biotechnology and Bioprocess Engineering
, Piotr Bąbik (FCPE)
Piotr Bąbik,,
- Faculty of Chemical and Process Engineering
, Barbara Marczak (FCPE)
Barbara Marczak,,
- Faculty of Chemical and Process Engineering
, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesInternational Journal of Artificial Organs, ISSN 0391-3988
Issue year2013
Vol36
No8
Pages576-577
Publication size in sheets0.3
ConferenceXL Congress of the European Society for Artificial Organs (Esao2013), 11-09-2013 - 14-09-2013, Glasgow, Wielka Brytania
Keywords in Englishblood coagulation, endothelial cell, endothelialization, hemocompatibility, polyurethane, REDV peptide, surface modification
ASJC Classification2204 Biomedical Engineering; 2502 Biomaterials; 2700 General Medicine; 2701 Medicine (miscellaneous); 1502 Bioengineering
DOIDOI:10.5301/ijao.5000244
URL http://www.artificial-organs.com/article/oral-presentations-and-posters---xl-annual-esao-congress---11-14-september-2013--glasgow--scotland--uk-
Languageen angielski
File
ButrukB.esao.pdf 242.8 KB
Score (nominal)20
Score sourcejournalList
ScoreMinisterial score = 20.0, 07-04-2020, ArticleFromJournalAndMatConfByConferenceseries
Ministerial score (2013-2016) = 20.0, 07-04-2020, ArticleFromJournalAndMatConfByConferenceseries
Publication indicators WoS Citations = 5; GS Citations = 8.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.599; WoS Impact Factor: 2013 = 1.448 (2) - 2013=1.454 (5)
Citation count*8 (2020-01-13)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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