Human mesenchymal stem cell (hMSC) differentiation towards cardiac cells using a new microbioanalytical method

Patrycja Sokołowska , Kamil Żukowski , Iwona Lasocka , Lidia Szulc-Dabrowska , Elżbieta Jastrzębska

Abstract

Stem cells (SCs) are more and more often applied in tissue engineering and cell therapies, e.g. in regenerative medicine. Standard methods of SC differentiation are time consuming and ineffective. Therefore, new bioanalytical methods (i.e. Lab-on-a-Chip systems) are develop to improve such type of studies. Although, microtechnology is a rapidly growing research area, there are so far not too many works which present SC differentiation into cardiomyocytes in the microsystems. Therefore, we present new microbioanalytical method of SC differentiation towards cardiac cells using a newly developed digitally controlled microdispenser integrated with a Heart-on-a-chip system. Seven-day culture of human mesenchymal stem cells (hMSCs) and their differentiation using biochemical factors such as 5-AZA (2 μM, 24 h) and VEGF (20 ng ml-1, 72 h) were investigated in the microsystem which was automatically operated using smartphone software. hMSC differentiation into the cardiac cells was confirmed using immunostaining of cardiac markers (α-actinin and troponin T). The usage of the microsystem allowed shortening the time of hMSC differentiation in comparison to macroscale method. We showed that the microsystem, in which the in vivo microenvironment is mimicked and dynamic conditions are provided by a microdispenser, favorably affect hMSC differentiation towards cardiac cells. Based on the presented research we can conclude that the developed digitally controlled microsystem could be successfully utilized as a new microbioanalytical method for stem cells differentiation and analysis of their function under dynamic conditions. In the future, this could be a helpful tool for scientists working on regenerative medicine.

Author Patrycja Sokołowska (FC / CMB)
Patrycja Sokołowska,,
- Chair of Medical Biotechnology
, Kamil Żukowski (CAMT)
Kamil Żukowski,,
- The Centre for Advanced Materials and Technologies
, Iwona Lasocka - [University of Life Sciences in Lublin]
Iwona Lasocka,,
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, Lidia Szulc-Dabrowska - [Szkola Glowna Gospodarstwa Wiejskiego]
Lidia Szulc-Dabrowska,,
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, Elżbieta Jastrzębska (FC / CMB)
Elżbieta Jastrzębska,,
- Chair of Medical Biotechnology
Journal seriesAnalyst, [The Analyst], ISSN 0003-2654, e-ISSN 1364-5528
Issue year2020
Vol145
Pages3017-3028
ASJC Classification1303 Biochemistry; 1602 Analytical Chemistry; 1603 Electrochemistry; 1607 Spectroscopy; 2304 Environmental Chemistry
DOIDOI:10.1039/c9an02366f
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 04-06-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 0.945; WoS Impact Factor: 2018 = 4.019 (2) - 2018=3.857 (5)
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