Electrochemical Studies on the Binding of Antibody-Aptamer Hybrid Receptor Layers to HER2 Protein

Marta Maria Jarczewska , Jessica Wieczorek , Elżbieta Malinowska


One of the possibilities of early detection of cancer disease is the analysis of the presence of protein biomarkers. Herein, we present the studies on the hybrid antibody- A ptamer receptor layers for electrochemical detection of HER2 protein that is known as a biomarker of e.g. breast cancer. The application of MB-labelled aptamer did not allow to evidence the interaction with HER2 protein. Moreover, the use of unlabelled aptamer as a capture element and polyclonal antibody as a reporter probe did not show a dependence of current signal change vs HER2 protein concentration as well. On the contrary, the application of monoclonal antibody as a capture probe and aptamer labelled at 3′ end with MB as reporter probe enabled the determination of HER2 within 0.01-10 ng•ml-1 concentration range. The elaborated affinity assay also showed good selectivity towards HER2 biomarker and was used for HER2 determination in spiked serum sample.

Author Marta Maria Jarczewska (FC / CMB)
Marta Maria Jarczewska,,
- Chair of Medical Biotechnology
, Jessica Wieczorek (FC / CMB)
Jessica Wieczorek,,
- Chair of Medical Biotechnology
, Elżbieta Malinowska (FC / CMB)
Elżbieta Malinowska,,
- Chair of Medical Biotechnology
Journal seriesJournal of the Electrochemical Society, ISSN 0013-4651, e-ISSN 1945-7111
Issue year2020
Publication size in sheets0.3
Article number067512
ASJC Classification1603 Electrochemistry; 2105 Renewable Energy, Sustainability and the Environment; 2504 Electronic, Optical and Magnetic Materials; 2505 Materials Chemistry; 2508 Surfaces, Coatings and Films; 3104 Condensed Matter Physics
URL https://iopscience.iop.org/article/10.1149/1945-7111/ab80ac
Languageen angielski
Jarczewska_2020_J._Electrochem._Soc._167_067512.pdf 769.1 KB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 07-08-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.009; WoS Impact Factor: 2018 = 3.12 (2) - 2018=3.405 (5)
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