Electrochemical Studies on the Binding of Antibody-Aptamer Hybrid Receptor Layers to HER2 Protein
Marta Maria Jarczewska , Jessica Wieczorek , Elżbieta Malinowska
One of the possibilities of early detection of cancer disease is the analysis of the presence of protein biomarkers. Herein, we present the studies on the hybrid antibody- A ptamer receptor layers for electrochemical detection of HER2 protein that is known as a biomarker of e.g. breast cancer. The application of MB-labelled aptamer did not allow to evidence the interaction with HER2 protein. Moreover, the use of unlabelled aptamer as a capture element and polyclonal antibody as a reporter probe did not show a dependence of current signal change vs HER2 protein concentration as well. On the contrary, the application of monoclonal antibody as a capture probe and aptamer labelled at 3′ end with MB as reporter probe enabled the determination of HER2 within 0.01-10 ng•ml-1 concentration range. The elaborated affinity assay also showed good selectivity towards HER2 biomarker and was used for HER2 determination in spiked serum sample.
|Journal series||Journal of the Electrochemical Society, ISSN 0013-4651, e-ISSN 1945-7111|
|Publication size in sheets||0.3|
|ASJC Classification||; ; ; ; ;|
|Score||= 100.0, 07-08-2020, ArticleFromJournal|
|Publication indicators||= 0; : 2017 = 1.009; : 2018 = 3.12 (2) - 2018=3.405 (5)|
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