Electrochemical Studies on the Binding of Antibody-Aptamer Hybrid Receptor Layers to HER2 Protein

Marta Maria Jarczewska , Jessica Wieczorek , Elżbieta Malinowska

Abstract

One of the possibilities of early detection of cancer disease is the analysis of the presence of protein biomarkers. Herein, we present the studies on the hybrid antibody- A ptamer receptor layers for electrochemical detection of HER2 protein that is known as a biomarker of e.g. breast cancer. The application of MB-labelled aptamer did not allow to evidence the interaction with HER2 protein. Moreover, the use of unlabelled aptamer as a capture element and polyclonal antibody as a reporter probe did not show a dependence of current signal change vs HER2 protein concentration as well. On the contrary, the application of monoclonal antibody as a capture probe and aptamer labelled at 3′ end with MB as reporter probe enabled the determination of HER2 within 0.01-10 ng•ml-1 concentration range. The elaborated affinity assay also showed good selectivity towards HER2 biomarker and was used for HER2 determination in spiked serum sample.

Author Marta Maria Jarczewska (FC / CMB)
Marta Maria Jarczewska,,
- Chair of Medical Biotechnology
, Jessica Wieczorek (FC / CMB)
Jessica Wieczorek,,
- Chair of Medical Biotechnology
, Elżbieta Malinowska (FC / CMB)
Elżbieta Malinowska,,
- Chair of Medical Biotechnology
Journal seriesJournal of the Electrochemical Society, ISSN 0013-4651, e-ISSN 1945-7111
Issue year2020
Vol167
No067512
Pages1-8
Publication size in sheets0.3
Article number067512
ASJC Classification1603 Electrochemistry; 2105 Renewable Energy, Sustainability and the Environment; 2504 Electronic, Optical and Magnetic Materials; 2505 Materials Chemistry; 2508 Surfaces, Coatings and Films; 3104 Condensed Matter Physics
DOIDOI:10.1149/1945-7111/ab80ac
URL https://iopscience.iop.org/article/10.1149/1945-7111/ab80ac
Languageen angielski
File
Jarczewska_2020_J._Electrochem._Soc._167_067512.pdf 769.1 KB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 07-08-2020, ArticleFromJournal
Publication indicators Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.009; WoS Impact Factor: 2018 = 3.12 (2) - 2018=3.405 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back
Confirmation
Are you sure?