Preparation of polysaccharide coated fluorescence nanocrystals for cancer diagnosis
Iga Wasiak , Tomasz Ciach
AbstractAccording to the WHO, cancer is the major cause of mortality, more than 8 million deaths annually, and this number is expected to rise. Cancer occurs over the years, from one normal cell via multistep carcinogenesis process entailing numerous cellular physiological system. This make it highly incomprehensible and complex disease. Despite the big international scientific effort cancer treatment remains one of the biggest challenges of contemporary medicine. There is no single test that can accurately diagnose cancer. The complete evaluation of a patient usually requires a thorough history and physical examination along with diagnostic testing. Many tests are needed to determine whether a person has cancer, or if another condition (such as an infection) is mimicking the symptoms of cancer. Many different tests exist to detect and measure almost any type of chemical component in blood or urine. Components may include blood glucose, electrolytes, enzymes, hormones, lipids (fats), other metabolic substances, and proteins. Endoscopic Confocal Microscopy (ECM) is probably one of the most important technological advances for the early detection of dysplastic lesions or adenocarcinoma within gastrointestinal tract, and for identification of risky fields suitable for targeted biopsies and a definitive histological evaluation. However, there is an urgent clinical need for increasing the detection sensitivity, and this depends on the development of new specific fluorescent probes. The aim of this study was to coated organic nanocrystals (NC) based on fluorescent dyes by a polysaccharide shell. These particles could display distinct advantages. Dye nanocrystals offer the highest dye concentration in the smallest volume, are particularly stable and allow fine tuning of the photophysical properties. The role of the polysaccharide shell is to limit crystal growth, protect the dye molecules from the outer medium, and avoid their dispersion before they have reached their target. Above all, it should make the particle less phagocyte-prone and ensure long circulation time in the blood stream. The coated nanocrystals could then enter the cancerous cells owing to the inherent leaky vasculature that serves cancerous tissues, and get concentrated there (EPR effect), quite selectively.
|Publication size in sheets||0.3|
|Book||VI Krajowa Konferencja Nanotechnologii, 2013, ul. Piastów 17, Szczecin 70-310, Przedsiębiorstwo Produkcyjno-Handlowe ZAPOL Dmochowski, Sobczyk, Sp. J., ISBN 978-83-7518-571-3, 181 p.|
|Keywords in Polish||nanokryształy fluorescencyjne, nanocząstki polisacharydowe|
|Keywords in English||fluorescence nanocrystal, polysaccharide nanoparticles|
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