Evaluation of nanoencapsulated verteporfin's cytotoxicity using a microfluidic system

Katarzyna Tokarska , Magdalena Bułka , Urszula Bazylińska , Elżbieta Jastrzębska , Michał Chudy , Artur Dybko , Kazimiera Anna Wilk , Zbigniew Brzózka


A new-generation of nanoencapsulated photosensitizers could be a good solution to perform effective photodynamic therapy (PDT). In this paper, we present physicochemical characterization and cellular investigation of newly prepared long-sustained release oil-core polyelectrolyte nanocarriers loaded with verteporfin (nano VP) in relation to free VP. For this purpose, a macroscale multiwell plates and multifunctional microfluidic system (for three types of cell cultures: monoculture, coculture and mixed culture) were used. A physical analysis of nano VP showed its high stability, monodispersity with unimodal shape and highly positive charge, what made them good candidates for cancer treatment. Biological properties (cellular internalization and uptake as well as cytotoxicity) of nano and free VP were evaluated using both carcinoma (A549) and normal (MRC-5) human lung cells. It was investigated that verteporfin was accumulated in cancer cells preferentially. Low cytotoxicity of the tested photosensitizer was observed in both macro, and microscale. However, in experiments performed in the microsystem, nano VP allowed the reduction of cytotoxic effect, especially in relation to the normal cells. It could result from the specific environment of cell growth in the microsystem which can quite closely mimic the in vivo conditions. Our results suggest that the presented microsystem could be a very useful microtool for testing of new generation of photosensitizers in various configurations of cell cultures, which are difficult to perform in the macroscale. Moreover, the prepared nano VP could be successfully used for further research i.e. evaluation of PDT procedures.
Author Katarzyna Tokarska ZMB
Katarzyna Tokarska,,
- Department Of Microbioanalytics
, Magdalena Bułka ZMB
Magdalena Bułka,,
- Department Of Microbioanalytics
, Urszula Bazylińska
Urszula Bazylińska,,
, Elżbieta Jastrzębska ZMB
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, Michał Chudy ZMB
Michał Chudy,,
- Department Of Microbioanalytics
, Artur Dybko ZMB
Artur Dybko,,
- Department Of Microbioanalytics
, Kazimiera Anna Wilk
Kazimiera Anna Wilk,,
, Zbigniew Brzózka ZMB
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesJournal of Pharmaceutical and Biomedical Analysis, ISSN 0731-7085
Issue year2016
Publication size in sheets0.5
Keywords in EnglishCytotoxicity; Lab-on-a-chip; Nanoencapsulated photosensitizers; Photodynamic therapy; Verteporfin
URL http://www.sciencedirect.com/science/article/pii/S0731708516301145
Languageen angielski
1-s2.0-S0731708516301145-main.pdf 2.62 MB
Score (nominal)35
ScoreMinisterial score [Punktacja MNiSW] = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 3.255 (2) - 2016=2.953 (5)
Citation count*5 (2018-06-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.