Fenton-type reaction grafting of polyvinylpyrrolidone onto polypropylene membrane for improving hemo- and biocompatibility

Ilona Łojszczyk , Aleksandra Kuźmińska , Beata Aleksandra Butruk-Raszeja , Tomasz Ciach

Abstract

Known techniques for modification of polypropylene membranes (PPm) often require modification of the membrane in its entire volume (i.e. at the manufacturing stage), which may affect its properties. In the present work, the authors proposed a simple method for PPm hydrophilization. The process involves a two-step Fenton-type reaction, with ethylene glycol dimethacrylate (EGDMA) as a crosslinking agent and cumene hydroperoxide (CHP) as a source of free radicals. This hydrogel coating aims to enhance membrane hemocompatible and biocompatible properties. The biggest advantage of the proposed technique is the change of materials' surface properties, without interfering with its internal structure. Microscopic (SEM) and spectroscopic (FTIR-ATR) analyses confirmed the presence of hydrogel coating on PPm surfaces. Additionally, the evaluation of the surface density of the coating showed that the thickness of the coating increases with the reaction time and CHP concentration. The applied coatings significantly increase surface hydrophilicity (contact angle for PPm: 128.58° ± 0.52°, for all modified surfaces <53.31° ± 2.03°). The cytotoxicity test (XTT assay) proved biocompatibility of the PVP coating – cell viability remained above 90% for all variants tested. The modification resulted in a decrease in fibrinogen adsorption (of at least about 16%) and in a number of surface-adhered platelets. The assay evaluating the amount of secreted cell adhesion molecules (ICAM-1) showed a significant reduction (of at least about 50%) in the expression of ICAM-1 for all hydrogel-modified surfaces.
Author Ilona Łojszczyk (FCPE / DBBE)
Ilona Łojszczyk,,
- Department of Biotechnology and Bioprocess Engineering
, Aleksandra Kuźmińska (FCPE / DBBE)
Aleksandra Kuźmińska,,
- Department of Biotechnology and Bioprocess Engineering
, Beata Aleksandra Butruk-Raszeja (FCPE / DBBE)
Beata Aleksandra Butruk-Raszeja,,
- Department of Biotechnology and Bioprocess Engineering
, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesMaterials Science & Engineering C - Materials for Biological Applications, [Materials Science and Engineering C], ISSN 0928-4931, e-ISSN 1873-0191
Issue year2020
Vol113
Pages1-11
Article number110960
Keywords in Englishpolypropylene, membrane, hydrogel, surface modification
ASJC Classification2210 Mechanical Engineering; 2211 Mechanics of Materials; 2500 General Materials Science; 3104 Condensed Matter Physics
DOIDOI:10.1016/j.msec.2020.110960
URL https://reader.elsevier.com/reader/sd/pii/S0928493119337749?token=8F8A9DAA7A68E0B61839BE4498A7A9DFF26014B83CF9E8814DFC5EFD9D247CF94132AED5FED391359113D8E5CDF358BD
Languageen angielski
File
Łojszczyk I. (i in.) - Fenton-type reaction...pdf 4.76 MB
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 22-09-2020, ArticleFromJournal
Publication indicators Scopus Citations = 1; GS Citations = 1.0; WoS Citations = 1; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.384; WoS Impact Factor: 2018 = 4.959 (2) - 2018=4.708 (5)
Citation count*1 (2020-09-07)
Additional fields
UwagiThe presented work has been supported by the project: “Study on hemocompatibility of modified polymer surfaces” – Preludium 8 - financed by The National Science Centre (grant agreement no. UMO-2014/15/N/ST8/02717), Poland. The authors would like to thank PhD Michał Wojasiński from the Warsaw University of Technology for performing the tensile strength tests and PhD Jakub W. Trzciński for the help with the water permeability tests.
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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