Internalization and cytotoxicity effects of carbon-encapsulated iron nanoparticles in murine endothelial cells : studies on internal dosages due to loaded mass agglomerates

Monika A. Cywińska , Michał Bystrzejewski , Magdalena Popławska , Anna Kośmider , Robert Zdanowski , Sławomir Lewicki , Zbigniew Fijalek , Agnieszka Ostrowska , Magdalena Bamburowicz , Andrzej Cieszanowski , Ireneusz P. Grudziński

Abstract

Carbon-encapsulated iron nanoparticles (CEINs) qualified asmetal-inorganic hybrid nanomaterials offer a potential scope for an increasing number of biomedical applications. In this study,we have focused on the investigation of cellular fate and resulting cytotoxic effects of CEINs synthesized using a carbon arc route and studied inmurine endothelial (HECa-10) cells. The CEIN samples were characterized as pristine (the mean diameter between 47 and 56 nm) and hydrodynamic (the mean diameter between 270 and 460 nm) forms and tested using a battery of methods to determine the cell internalization extent and cytotoxicity effects upon to the exposures (0.0001– 100 μg/ml) in HECa-10 cells. Our studies evidenced that the incubation with CEINs for 24 h is accompanied with substantial changes of Zeta potential in cells which can be considered as a key factor for affecting the membrane transport, cellular distribution and cytotoxicity of these nanoparticles. The results demonstrate that CEINs have entered the endothelial cell through the endocytic pathway rather than by passive diffusion and they were mainly loaded as agglomerates on the cell membrane and throughout the cytoplasm, mitochondria and nucleus. The studies show that CEINs induce the mitochondrial and cell membrane cytotoxicities in a dose-dependent manner resulting from the internal dosages due to CEIN agglomerates. Our results highlight the importance of the physicochemical characterization of CEINs in studying the magnetic nanoparticle–endothelial cell interactions because the CEIN mass agglomerates can sediment more or less rapidly in culture models.
Author Monika A. Cywińska - [Medical University of Warsaw]
Monika A. Cywińska,,
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, Michał Bystrzejewski - [University of Warsaw (UW)]
Michał Bystrzejewski,,
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- Uniwersytet Warszawski
, Magdalena Popławska (FC / DOC)
Magdalena Popławska,,
- Department Of Organic Chemistry
, Anna Kośmider - [Medical University of Warsaw]
Anna Kośmider,,
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, Robert Zdanowski - [Wojskowy Instytut Higieny i Epidemiologii imienia Generała Karola Kaczkowskiego(WIHiE), Warsaw]
Robert Zdanowski,,
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, Sławomir Lewicki - [Wojskowy Instytut Higieny i Epidemiologii imienia Generała Karola Kaczkowskiego(WIHiE), Warsaw]
Sławomir Lewicki,,
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, Zbigniew Fijalek - [Medical University of Warsaw]
Zbigniew Fijalek,,
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, Agnieszka Ostrowska - [Szkola Glowna Gospodarstwa Wiejskiego]
Agnieszka Ostrowska,,
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, Magdalena Bamburowicz - [Medical University of Warsaw]
Magdalena Bamburowicz,,
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, Andrzej Cieszanowski - [Medical University of Warsaw]
Andrzej Cieszanowski,,
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et al.`
Journal seriesToxicology in Vitro, ISSN 0887-2333, (A 30 pkt)
Issue year2016
Vol34
Pages229-236
Publication size in sheets0.3
Keywords in EnglishIron-carbon (core-shell) type hybrid nanoparticles; Endothelial (HECa-10) cells; Agglomerates; Internalization; Zeta potential; Cytotoxicity
ASJC Classification3005 Toxicology; 2700 General Medicine
DOIDOI:10.1016/j.tiv.2016.04.011
URL http://www.sciencedirect.com/science/article/pii/S0887233316300789
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_2847_org.pdf 2.5 MB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 03-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 03-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 5; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 0.941; WoS Impact Factor: 2016 = 2.866 (2) - 2016=3.103 (5)
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