Internalization and cytotoxicity effects of carbon-encapsulated iron nanoparticles in murine endothelial cells : studies on internal dosages due to loaded mass agglomerates

Monika A. Cywińska , Michał Bystrzejewski , Magdalena Popławska , Anna Kośmider , Robert Zdanowski , Sławomir Lewicki , Zbigniew Fijalek , Agnieszka Ostrowska , Magdalena Bamburowicz , Andrzej Cieszanowski , Ireneusz P. Grudziński

Abstract

Carbon-encapsulated iron nanoparticles (CEINs) qualified asmetal-inorganic hybrid nanomaterials offer a potential scope for an increasing number of biomedical applications. In this study,we have focused on the investigation of cellular fate and resulting cytotoxic effects of CEINs synthesized using a carbon arc route and studied inmurine endothelial (HECa-10) cells. The CEIN samples were characterized as pristine (the mean diameter between 47 and 56 nm) and hydrodynamic (the mean diameter between 270 and 460 nm) forms and tested using a battery of methods to determine the cell internalization extent and cytotoxicity effects upon to the exposures (0.0001– 100 μg/ml) in HECa-10 cells. Our studies evidenced that the incubation with CEINs for 24 h is accompanied with substantial changes of Zeta potential in cells which can be considered as a key factor for affecting the membrane transport, cellular distribution and cytotoxicity of these nanoparticles. The results demonstrate that CEINs have entered the endothelial cell through the endocytic pathway rather than by passive diffusion and they were mainly loaded as agglomerates on the cell membrane and throughout the cytoplasm, mitochondria and nucleus. The studies show that CEINs induce the mitochondrial and cell membrane cytotoxicities in a dose-dependent manner resulting from the internal dosages due to CEIN agglomerates. Our results highlight the importance of the physicochemical characterization of CEINs in studying the magnetic nanoparticle–endothelial cell interactions because the CEIN mass agglomerates can sediment more or less rapidly in culture models.
Author Monika A. Cywińska
Monika A. Cywińska,,
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, Michał Bystrzejewski
Michał Bystrzejewski,,
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, Magdalena Popławska ZChO
Magdalena Popławska,,
- Department Of Organic Chemistry
, Anna Kośmider
Anna Kośmider,,
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, Robert Zdanowski
Robert Zdanowski,,
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, Sławomir Lewicki
Sławomir Lewicki,,
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, Zbigniew Fijalek
Zbigniew Fijalek,,
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, Agnieszka Ostrowska
Agnieszka Ostrowska,,
-
, Magdalena Bamburowicz
Magdalena Bamburowicz,,
-
, Andrzej Cieszanowski
Andrzej Cieszanowski,,
-
et al.`
Journal seriesToxicology in Vitro, ISSN 0887-2333
Issue year2016
Vol34
Pages229-236
Publication size in sheets0.3
Keywords in EnglishIron-carbon (core-shell) type hybrid nanoparticles; Endothelial (HECa-10) cells; Agglomerates; Internalization; Zeta potential; Cytotoxicity
DOIDOI:10.1016/j.tiv.2016.04.011
URL http://www.sciencedirect.com/science/article/pii/S0887233316300789
Languageen angielski
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wdpb_publikacje_pliki_plik_publikacja_2847_org.pdf 2.5 MB
Score (nominal)30
ScoreMinisterial score [Punktacja MNiSW] = 30.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) [Punktacja MNiSW (2013-2016)] = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor [Impact Factor WoS]: 2016 = 2.866 (2) - 2016=3.103 (5)
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