Internalization and cytotoxicity effects of carbon-encapsulated iron nanoparticles in murine endothelial cells : studies on internal dosages due to loaded mass agglomerates
Monika A. Cywińska , Michał Bystrzejewski , Magdalena Popławska , Anna Kośmider , Robert Zdanowski , Sławomir Lewicki , Zbigniew Fijalek , Agnieszka Ostrowska , Magdalena Bamburowicz , Andrzej Cieszanowski , Ireneusz P. Grudziński
AbstractCarbon-encapsulated iron nanoparticles (CEINs) qualified asmetal-inorganic hybrid nanomaterials offer a potential scope for an increasing number of biomedical applications. In this study,we have focused on the investigation of cellular fate and resulting cytotoxic effects of CEINs synthesized using a carbon arc route and studied inmurine endothelial (HECa-10) cells. The CEIN samples were characterized as pristine (the mean diameter between 47 and 56 nm) and hydrodynamic (the mean diameter between 270 and 460 nm) forms and tested using a battery of methods to determine the cell internalization extent and cytotoxicity effects upon to the exposures (0.0001– 100 μg/ml) in HECa-10 cells. Our studies evidenced that the incubation with CEINs for 24 h is accompanied with substantial changes of Zeta potential in cells which can be considered as a key factor for affecting the membrane transport, cellular distribution and cytotoxicity of these nanoparticles. The results demonstrate that CEINs have entered the endothelial cell through the endocytic pathway rather than by passive diffusion and they were mainly loaded as agglomerates on the cell membrane and throughout the cytoplasm, mitochondria and nucleus. The studies show that CEINs induce the mitochondrial and cell membrane cytotoxicities in a dose-dependent manner resulting from the internal dosages due to CEIN agglomerates. Our results highlight the importance of the physicochemical characterization of CEINs in studying the magnetic nanoparticle–endothelial cell interactions because the CEIN mass agglomerates can sediment more or less rapidly in culture models.
|Journal series||Toxicology in Vitro, ISSN 0887-2333|
|Publication size in sheets||0.3|
|Keywords in English||Iron-carbon (core-shell) type hybrid nanoparticles; Endothelial (HECa-10) cells; Agglomerates; Internalization; Zeta potential; Cytotoxicity|
|Score|| = 30.0, 03-02-2020, ArticleFromJournal|
= 30.0, 03-02-2020, ArticleFromJournal
|Publication indicators||= 5; = 3; : 2016 = 0.941; : 2016 = 2.866 (2) - 2016=3.103 (5)|
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