Effect of triterpene and steroid saponins on lecithin bilayers

Kamil Wojciechowski , Marta Orczyk , Marcus Trapp , Thomas Gutberlet

Abstract

We describe the effect of four triterpene (α-hederin, hederacoside C, ammonium glycyrrhizate, Quillaja bark saponins (QBS)) and one steroid (digitonin) saponins on lecithin and lecithin-cholesterol (4:1. mol/mol) model bilayers. For this purpose the Giant Unilamellar Vesicles (GUV), Large Unilamellar Vesicles (LUV) and Supported Lipid Bilayers (SLB) were probed with fluorescence microscopy, dynamic light scattering and neutron reflectivity, respectively. In most cases the results provided from the three techniques were consistent. In cholesterol-free systems, only α-hederin was able to penetrate SLB and induce macroscopic changes in LUV and GUV. Digitonin can adsorb to a small extent on the surface of cholesterol-free SLB, but does not affect macroscopically the LUV and GUV. In the presence of cholesterol, not only α-hederin but also QBS and digitonin affect the liposomes both microscopically and macroscopically. The present data clearly confirm that cholesterol significantly enhances the effect of membrane-active saponins on bilayers and that the effect on model bilayers correlates well with the membrane-activity of the saponins.
Author Kamil Wojciechowski (FC / IBC / DM)
Kamil Wojciechowski,,
- Department Of Microbioanalytics
, Marta Orczyk (FC / IBC / DM)
Marta Orczyk,,
- Department Of Microbioanalytics
, Marcus Trapp
Marcus Trapp,,
-
, Thomas Gutberlet
Thomas Gutberlet,,
-
Journal seriesColloids and Surfaces A-Physicochemical and Engineering Aspects, ISSN 0927-7757
Issue year2016
Vol510
Pages150-158
Publication size in sheets0.5
Keywords in EnglishBiosurfactant; Fluorescence microscopy; GUV; Liposome; Neutron reflectivity
ASJC Classification1505 Colloid and Surface Chemistry
DOIDOI:10.1016/j.colsurfa.2016.04.065
URL http://www.sciencedirect.com/science/article/pii/S0927775716303107
Languageen angielski
File
1-s2.0-S0927775716303107-main.pdf 2.53 MB
Score (nominal)30
Score sourcejournalList
ScoreMinisterial score = 30.0, 01-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 01-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 5; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.136; WoS Impact Factor: 2016 = 2.714 (2) - 2016=2.838 (5)
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