Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine-type photosensitizers

Elżbieta Jastrzębska , Urszula Bazylińska , Magdalena Bułka , Katarzyna Tokarska , Michał Chudy , Artur Dybko , Kazimiera Anna Wilk , Zbigniew Brzózka

Abstract

he application of nanotechnology is important to improve research and development of alternative anticancer therapies. In order to accelerate research related to cancer diagnosis and to improve the effectiveness of cancer treatment, various nanomaterials are being tested. The main objective of this work was basic research focused on examination of the mechanism and effectiveness of the introduction of nanoencapsulated photosensitizers to human carcinoma (A549) and normal cells (MRC-5). Newly encapsulated hydrophobic indocyanine-type photosensitizer (i.e., IR-780) was subjected to in vitro studies to determine its release characteristics on a molecular level. The photosensitizers were delivered to carcinoma and normal cells cultured under model conditions using multiwell plates and with the use of the specially designed hybrid (poly(dimethylsiloxane) (PDMS)/glass) microfluidic system. The specific geometry of our microsystem allows for the examination of intercellular interactions between cells cultured in the microchambers connected with microchannels of precisely defined length. Our microsystem allows investigating various therapeutic procedures (e.g., photodynamic therapy) on monoculture, coculture, and mixed culture, simultaneously, which is very difficult to perform using standard multiwell plates. In addition, we tested the cellular internalization of nanoparticles (differing in size, surface properties) in carcinoma and normal lung cells. We proved that cellular uptake of nanocapsules loaded with cyanine IR-780 in carcinoma cells was more significant than in normal cells. We demonstrated non cytotoxic effect of newly synthesized nanocapsules built with polyelectrolytes (PEs) of opposite surface charges: polyanion-polysodium-4-styrenesulphonate and polycation-poly(diallyldimethyl-ammonium) chloride loaded with cyanine IR-780 on human lung carcinoma and normal cell lines. However, the differences observed in the photocytotoxic effect between two types of tested nanocapsules can result from the type of last PE layer and their different surface charge.
Author Elżbieta Jastrzębska ZMB
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, Urszula Bazylińska
Urszula Bazylińska,,
-
, Magdalena Bułka ZMB
Magdalena Bułka,,
- Department Of Microbioanalytics
, Katarzyna Tokarska ZMB
Katarzyna Tokarska,,
- Department Of Microbioanalytics
, Michał Chudy ZMB
Michał Chudy,,
- Department Of Microbioanalytics
, Artur Dybko ZMB
Artur Dybko,,
- Department Of Microbioanalytics
, Kazimiera Anna Wilk
Kazimiera Anna Wilk,,
-
, Zbigniew Brzózka ZMB
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesBiomicrofluidics, ISSN 1932-1058
Issue year2016
Vol10
Pages014116_1-014116_15
Publication size in sheets0.7
Keywords in EnglishBiological organs; Cell culture; Cells; Cytology; Cytotoxicity; Diseases; Microchannels; Microfluidics; Microsystems; Nanocapsules; Photodynamic therapy; Polyelectrolytes Anti-cancer therapies; Cellular internalization; Intercellular interactions; Microfluidic platforms; Polydimethylsiloxane PDMS; Release characteristics; Research and development; Therapeutic procedures
DOIDOI:10.1063/1.4941681
URL http://scitation.aip.org/content/aip/journal/bmf/10/1/10.1063/1.4941681
Languageen angielski
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Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine.pdf (file archived - login or check accessibility on faculty) Microfluidic platform for photodynamic therapy cytotoxicity analysis of nanoencapsulated indocyanine.pdf 3.12 MB
Score (nominal)35
ScoreMinisterial score = 30.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.535 (2) - 2016=3.105 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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