Nanoconjugates of ferrocene and carbon-encapsulated iron nanoparticles as sensing platforms for voltammetric determination of ceruloplasmin in blood

Jakub Sęk , Artur Kasprzak , Michał Bystrzejewski , Magdalena Popławska , Waldemar Kaszuwara , Zbigniew Stojek , Anna M. Nowicka

Abstract

The nanoparticles comprising of iron core and carbon shell were decorated with ferrocene derivatives: ferrocene ecarboxaldehyde(Fc-1) and ferrocene ecarboxaldehyde oxime (Fc-2). A microdrop of suspension of the nanoconjugatewas placed on a glassy-carbon electrode to prepare the recognition/sensing layer. Drying and purification of the sensing layer resulted in a well-defined and stable square-wave voltammogram of the ferrocene moiety. The height of the voltammetric peak increased in the presence of ceruloplasmin. That increase was linearly dependent on the logarithmic concentration of ceruloplasmin in blood. The applied external magnetic field was a factor which yielded better sensitivity and repeatability of the sensor response. The linearity of sensor response was found to be between 0.001 and 10 μg dL−1 and 0.05–10 μg dL−1 for both nanoconjugates: Fe@C-Fc-1 and Fe@C-Fc-2, in the presence and absence of the magnet, respectively. The obtained detection limit (LOD) for Fe@C-Fc-1 was found to be 0.60 and 0.10 μg dL−1 in the absence and presence of magnetic field, respectively, whilst for Fe@C-Fc-2 was 0.4 and 0.07 μg dL−1 in the absence and presence of a magnet, respectively. The proposed method is selective because the presence of common antioxidants in blood did not interfere significantly with the determination of the concentration of ceruloplasmin.
Author Jakub Sęk - Faculty of Chemistry (WChUW) [Uniwersytet Warszawski (UW)]
Jakub Sęk,,
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, Artur Kasprzak (FC / DOC)
Artur Kasprzak,,
- Department Of Organic Chemistry
, Michał Bystrzejewski - Faculty of Chemistry (WChUW) [Uniwersytet Warszawski (UW)]
Michał Bystrzejewski,,
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, Magdalena Popławska (FC / DOC)
Magdalena Popławska,,
- Department Of Organic Chemistry
, Waldemar Kaszuwara (FMSE / DCFM)
Waldemar Kaszuwara,,
- Division of Construction and Functional Materials
, Zbigniew Stojek - Faculty of Chemistry (WChUW) [Uniwersytet Warszawski (UW)]
Zbigniew Stojek,,
-
, Anna M. Nowicka - Faculty of Chemistry (WChUW) [Uniwersytet Warszawski (UW)]
Anna M. Nowicka,,
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Journal seriesBiosensors & Bioelectronics, ISSN 0956-5663, (A 40 pkt)
Issue year2018
Vol102
Pages490-496
Publication size in sheets0.5
Keywords in EnglishNanoconjugates, Ferrocene, Carbon-encapsulated iron nanoparticles, Ceruloplasmin, Voltammetry, Blood analysis
DOIDOI:10.1016/j.bios.2017.11.060
Languageen angielski
File
Biosensors 102(20918)490-496.pdf 1.04 MB
Score (nominal)40
ScoreMinisterial score = 40.0, 07-03-2018, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 07-03-2018, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 7.78 (2) - 2016=6.862 (5)
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