Internal nanocrystalline structure and stiffness alterations of electrospun polycaprolactone-based mats after six months of in vitro degradation. An atomic force microscopy assay

Adrian Chlanda , Ewa Kijeńska-Gawrońska , Joanna Zdunek , Wojciech Święszkowski

Abstract

Biodegradable electrospun nanofibrous scaffolds for bone tissue engineering applications have been extensively studied as they can provide attractive open-worked architecture resembling natural extracellular matrix, with tunable physical and mechanical properties enhancing positive cellular response. For this purpose, electrospun mats were tested in terms of morphology, mechanical and physical properties, degradation kinetics and related phenomena occurring in micro- and nanoscale. However, detailed description of internal nanostructures of electrospun mats and their changes related to in vitro degradation is still missing. In this manuscript, we report qualitative and quantitative evaluation of internal lamellar nanostructure of electrospun fibrous scaffolds made of pristine polycaprolactone and composite with polymeric matrix and nanoceramic (hydroxyapatite) filler during in vitro degradation. Morphological and mechanical studies performed with an atomic force microscope were followed by scanning electron microscope imaging and X-Ray diffraction. The results suggest degradation-dependent alteration of both organization and thickness of nano-scaled lamellas recorded with atomic force microscope. Moreover, changes of the material's internal structure were followed by enhanced stiffness and higher crystallinity of electrospun fibers.

Author Adrian Chlanda (FMSE / DMD)
Adrian Chlanda,,
- Division of Materials Design
, Ewa Kijeńska-Gawrońska (FMSE / DMD)
Ewa Kijeńska-Gawrońska,,
- Division of Materials Design
, Joanna Zdunek (FMSE / DMD)
Joanna Zdunek,,
- Division of Materials Design
, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
Journal seriesJournal of the Mechanical Behavior of Biomedical Materials, ISSN 1751-6161, e-ISSN 1878-0180, (N/A 100 pkt)
Issue year2020
Vol101
Pages1-9
Publication size in sheets0.5
Article number103437
Keywords in Englishnanomaterial; biomaterial; mechanical properties; atomic force microscopy
ASJC Classification2204 Biomedical Engineering; 2211 Mechanics of Materials; 2502 Biomaterials
DOIDOI:10.1016/j.jmbbm.2019.103437
URL https://doi.org/10.1016/j.jmbbm.2019.103437
Languageen angielski
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Internal nanocrystalline structure and stiffness alterations of electrospun polycaprolactone-based mats after six months of in vitro degradation. An atomic force microscopy assay.pdf 3.08 MB
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 26-11-2019, ArticleFromJournal
Publication indicators Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.48; WoS Impact Factor: 2018 = 3.485 (2) - 2018=3.643 (5)
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