SEC ICP MS and CZE ICP MS investigation of medium and high molecular weight complexes formed by cadmium ions with phytochelatins

Agata Miszczak , Magdalena Rosłon , Grzegorz Zbroja , Katarzyna Brama , Elżbieta Szalacha , Helena Gawrońska , Katarzyna Pawlak

Abstract

Size-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) coupled with inductively coupled plasma mass spectrometry were applied to characterize low, medium, and high molecular weight cadmium complexes with glutathione and phytochelatins (PCs). The dominant stoichiometry of the complexes formed in vitro was established as 1:1 using electrospray ionization mass spectrometry. Calculated molecular masses of Cd1L1 complexes were used for calibration of the SEC and CZE methods. The results showed a lower (2 kDa) SEC column exclusion limit for cadmium complexes compared with free peptides (10 kDa), and most of the high molecular weight cadmium species were eluted in the void volume of the column. Moreover, the CZE method based on the semiempirical model of Offord to elucidate peptide migration allowed us to show a high propensity of Cd-PC complexes for polymorphism on complexation, which was also observed for extracts of Arabidopsis thaliana treated with cadmium. All the information presented is vital for understanding the mechanism of metal deactivation in plants.
Author Agata Miszczak (FC / CAC)
Agata Miszczak,,
- Chair Of Analytical Chemistry
, Magdalena Rosłon (FC / CAC)
Magdalena Rosłon,,
- Chair Of Analytical Chemistry
, Grzegorz Zbroja (FC / CAC)
Grzegorz Zbroja,,
- Chair Of Analytical Chemistry
, Katarzyna Brama (FC / CAC)
Katarzyna Brama,,
- Chair Of Analytical Chemistry
, Elżbieta Szalacha - [Szkola Glowna Gospodarstwa Wiejskiego]
Elżbieta Szalacha,,
-
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, Helena Gawrońska - [Szkola Glowna Gospodarstwa Wiejskiego]
Helena Gawrońska,,
-
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, Katarzyna Pawlak (FC / CAC)
Katarzyna Pawlak,,
- Chair Of Analytical Chemistry
Journal seriesAnalytical and Bioanalytical Chemistry, ISSN 1618-2642, [1618-2650]
Issue year2013
Vol405
No14
Pages4667-4678
Publication size in sheets0.55
Keywords in EnglishPhytochelatins; Cadmium complexes; Capillary zone electrophoresis; inductively coupled plasma; mass spectrometry; Size-exclusion chromatography; inductively coupled plasma mass spectrometry; Electrospray ionization mass spectrometry
ASJC Classification1303 Biochemistry; 1602 Analytical Chemistry
DOIDOI:10.1007/s00216-013-6868-3
URL http://link.springer.com/article/10.1007%2Fs00216-013-6868-3
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_1954_org.pdf 502.2 KB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 01-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 01-02-2020, ArticleFromJournal
Publication indicators Scopus Citations = 15; WoS Citations = 14; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.222; WoS Impact Factor: 2013 = 3.578 (2) - 2013=3.744 (5)
Citation count*7 (2015-05-10)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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