SEC ICP MS and CZE ICP MS investigation of medium and high molecular weight complexes formed by cadmium ions with phytochelatins
Agata Miszczak , Magdalena Rosłon , Grzegorz Zbroja , Katarzyna Brama , Elżbieta Szalacha , Helena Gawrońska , Katarzyna Pawlak
AbstractSize-exclusion chromatography (SEC) and capillary zone electrophoresis (CZE) coupled with inductively coupled plasma mass spectrometry were applied to characterize low, medium, and high molecular weight cadmium complexes with glutathione and phytochelatins (PCs). The dominant stoichiometry of the complexes formed in vitro was established as 1:1 using electrospray ionization mass spectrometry. Calculated molecular masses of Cd1L1 complexes were used for calibration of the SEC and CZE methods. The results showed a lower (2 kDa) SEC column exclusion limit for cadmium complexes compared with free peptides (10 kDa), and most of the high molecular weight cadmium species were eluted in the void volume of the column. Moreover, the CZE method based on the semiempirical model of Offord to elucidate peptide migration allowed us to show a high propensity of Cd-PC complexes for polymorphism on complexation, which was also observed for extracts of Arabidopsis thaliana treated with cadmium. All the information presented is vital for understanding the mechanism of metal deactivation in plants.
|Journal series||Analytical and Bioanalytical Chemistry, ISSN 1618-2642, [1618-2650]|
|Publication size in sheets||0.55|
|Keywords in English||Phytochelatins; Cadmium complexes; Capillary zone electrophoresis; inductively coupled plasma; mass spectrometry; Size-exclusion chromatography; inductively coupled plasma mass spectrometry; Electrospray ionization mass spectrometry|
|Score|| = 40.0, 01-02-2020, ArticleFromJournal|
= 40.0, 01-02-2020, ArticleFromJournal
|Publication indicators||= 15; = 14; : 2014 = 1.222; : 2013 = 3.578 (2) - 2013=3.744 (5)|
|Citation count*||7 (2015-05-10)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.