Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering
Marco Costantini , Jan Guzowski , Paweł J. Żuk , Pamela Mozetic , Simone De Panfilis , Jakub Jaroszewicz , Marcin Heljak , Mara Massimi , Maxime Pierrone , Marcella Trombetta , Mariella Dentini , Wojciech Święszkowski , Alberto Rainer , Piotr Garstecki , Andrea Barbetta
AbstractInjection of cell‐laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range ≈300–1500 mm) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil‐in‐water emulsion inside a flow‐focusing microfluidic device. This emulsion is later broken‐up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.
|Journal series||Advanced Functional Materials, ISSN 1616-301X|
|Publication size in sheets||0.6|
|Keywords in English||cell carriers emulsion templating, high internal phase emulsion (HIPE), microfluidics, porous microbeads|
|ASJC Classification||; ; ;|
|Score||= 45.0, 17-09-2020, ArticleFromJournal|
|Publication indicators||= 12; = 4; : 2018 = 2.347; : 2018 = 15.621 (2) - 2018=14.755 (5)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.