Electric Field Assisted Microfluidic Platform for Generation of Tailorable Porous Microbeads as Cell Carriers for Tissue Engineering

Marco Costantini , Jan Guzowski , Paweł J. Żuk , Pamela Mozetic , Simone De Panfilis , Jakub Jaroszewicz , Marcin Heljak , Mara Massimi , Maxime Pierrone , Marcella Trombetta , Mariella Dentini , Wojciech Święszkowski , Alberto Rainer , Piotr Garstecki , Andrea Barbetta

Abstract

Injection of cell‐laden scaffolds in the form of mesoscopic particles directly to the site of treatment is one of the most promising approaches to tissue regeneration. Here, a novel and highly efficient method is presented for preparation of porous microbeads of tailorable dimensions (in the range ≈300–1500 mm) and with a uniform and fully interconnected internal porous texture. The method starts with generation of a monodisperse oil‐in‐water emulsion inside a flow‐focusing microfluidic device. This emulsion is later broken‐up, with the use of electric field, into mesoscopic double droplets, that in turn serve as a template for the porous microbeads. By tuning the amplitude and frequency of the electric pulses, the template droplets and the resulting porous bead scaffolds are precisely produced. Furthermore, a model of pulsed electrodripping is proposed that predicts the size of the template droplets as a function of the applied voltage. To prove the potential of the porous microbeads as cell carries, they are tested with human mesenchymal stem cells and hepatic cells, with their viability and degree of microbead colonization being monitored. Finally, the presented porous microbeads are benchmarked against conventional microparticles with nonhomogenous internal texture, revealing their superior performance.
Author Marco Costantini - Università Campus Bio-Medico di Roma, Rome, Italy [Universita degli Studi di Roma La Sapienza]
Marco Costantini,,
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, Jan Guzowski - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Institute of Physical Chemistry of the Polish Academy of Sciences]
Jan Guzowski,,
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, Paweł J. Żuk - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Princeton University]
Paweł J. Żuk,,
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, Pamela Mozetic - Università Campus Bio-Medico di Roma, Rome, Italy [Università Campus Bio-Medico di Roma]
Pamela Mozetic,,
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, Simone De Panfilis - Istituto Italiano di Tecnologia, Rome [Istituto Italiano di Tecnologia]
Simone De Panfilis,,
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, Jakub Jaroszewicz (FMSE / DMD)
Jakub Jaroszewicz,,
- Division of Materials Design
, Marcin Heljak (FMSE / DMD)
Marcin Heljak,,
- Division of Materials Design
, Mara Massimi - University of L'Aquila, L'Aquila, Italy [Università degli Studi dell'Aquila]
Mara Massimi,,
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, Maxime Pierrone - Telecom Physique Strasbourg
Maxime Pierrone,,
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, Marcella Trombetta - Università Campus Bio-Medico di Roma, Rome, Italy [Università Campus Bio-Medico di Roma]
Marcella Trombetta,,
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et al.`
Journal seriesAdvanced Functional Materials, ISSN 1616-301X, (A 45 pkt)
Issue year2018
Vol28
No20
Pages1800874 (1-13)
Publication size in sheets0.6
Keywords in English cell carriers emulsion templating, high internal phase emulsion (HIPE), microfluidics, porous microbeads
ASJC Classification1603 Electrochemistry; 3104 Condensed Matter Physics; 2502 Biomaterials; 2504 Electronic, Optical and Magnetic Materials
DOIDOI:10.1002/adfm.201800874
URL https://doi.org/10.1002/adfm.201800874
Languageen angielski
File
Costantini_et_al-2018-Advanced_Functional_Materials.pdf 2.47 MB
Score (nominal)45
ScoreMinisterial score = 45.0, 24-05-2019, ArticleFromJournal
Publication indicators Scopus Citations = 3; WoS Citations = 3; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 2.231; WoS Impact Factor: 2017 = 13.325 (2) - 2017=13.274 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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