Application of chromatography and mass spectrometry to the characterization of cobalt, copper, manganese and molybdenum in Morinda Citrifolia

Justyna Rybak , Lena Ruzik


An analytical procedure was proposed to determine the manganese species and to study the fractionation of microelements such as copper, cobalt and molybdenum in Noni juice. Morinda citrifolia is known as a noni fruit, Indian mulberry, nunaakai, dog dumpling, mengkudu, beach mulberry, vomit fruit and cheese fruit. It is a tropical plant with a long tradition of medicinal use in Polynesia and tropical parts of eastern Asia and Australia. This article covers the determination of manganese species in Noni juice and established by fractionation by size exclusion chromatography inductively coupled plasma mass spectrometry (SEC ICP MS) and next characterization of species by electrospray ionization mass spectrometry (ESI MS). Also presented the fractionation analysis of copper, cobalt and molybdenum in Noni juice sample using SEC ICP MS – juice was treated with buffer and enzymatic extraction media and analyzed. For the evaluation of the amounts of the metal fractions distinguished, the ICP MS was used off-line prior to the determination of copper, cobalt, molybdenum and manganese concentrations in the juice. It was established that elements are present in the analyzed samples in different species and their concentration is μg mL−1 and ng mL−1 range in fruit. The accuracy of the entire fractionation scheme and sample preparation procedures involved was verified by the performance of the recovery test. For the information about the bioavailability of these elements, in vitro bioavailability investigation was used by SEC ICP MS technique. Two step digestion model simulating gastric (pepsin digestion) and intestinal (pancreatin digestion) juices. In Noni juice, manganese is complexed from flavonoids – rutin, from dye like anthraquinone (alizarin) and glycosides – asperulosidic acid (ESI MS – characterization). The study shows that copper and molybdenum contained in Noni juice are complexed by peptides, and cobalt by organic acids (which are 3.6% of juice). Molybdenum in the sample is also bound by the polysaccharides (SEC ICP MS). In addition, compounds complexing manganese, copper and molybdenum are hydrophobic proteins.
Author Justyna Rybak (FC / CAC)
Justyna Rybak,,
- Chair Of Analytical Chemistry
, Lena Ruzik (FC / CAC)
Lena Ruzik,,
- Chair Of Analytical Chemistry
Journal seriesJournal of Chromatography A, ISSN 0021-9673
Issue year2013
Publication size in sheets0.5
Keywords in EnglishMorinda Citrifolia, Copper, Manganese, Cobalt, ICP MS, Bioavailability
ASJC Classification1308 Clinical Biochemistry; 1313 Molecular Medicine; 1602 Analytical Chemistry; 1605 Organic Chemistry; 2700 General Medicine; 1303 Biochemistry
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_1669_org.pdf 1.03 MB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 40.0, 17-08-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 17-08-2020, ArticleFromJournal
Publication indicators WoS Citations = 14; GS Citations = 9.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 1.615; WoS Impact Factor: 2013 = 4.258 (2) - 2013=4.339 (5)
Citation count*9 (2016-05-06)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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