A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells

Elżbieta Jastrzębska , Sylwia Flis , Aleksandra Rakowska , Michał Chudy , Zenon Jastrzębski , Artur Dybko , Zbigniew Brzózka

Abstract

We have investigated the response of normal and cancer cells to exposure a combination of celecoxib (Celbx) and 5-fluorouracil (5-FU) using a lab-on-a-chip microfluidic device. Specifically, we have tested the cytotoxic effect of Celbx on normal mouse embryo cells (Balb/c 3T3) and human lung carcinoma cells (A549). The single drugs or their combinations were adjusted to five different concentrations using a concentration gradient generator (CGG) in a single step. The results suggest that Celbx can enhanced the anticancer activity of 5-FU by stronger inhibition of cancer cell growth. We also show that the A549 cancer cells are more sensitive to Celbx than the Balb/c 3T3 normal cells. The results obtained with the microfluidic system were compared to those obtained with a macroscale in vitro cell culture method. In our opinion, the microfluidic system represents a unique approach for an evaluation of cellular response to multidrug exposure that also is more simple than respective microwell plate assays.
Author Elżbieta Jastrzębska (FC / IBC / DM)
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, Sylwia Flis - [National Medicines Institute, Warsaw]
Sylwia Flis,,
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, Aleksandra Rakowska (FC / IBC / DM)
Aleksandra Rakowska,,
- Department Of Microbioanalytics
, Michał Chudy (FC / IBC / DM)
Michał Chudy,,
- Department Of Microbioanalytics
, Zenon Jastrzębski - [National Medicines Institute, Warsaw]
Zenon Jastrzębski,,
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, Artur Dybko (FC / IBC / DM)
Artur Dybko,,
- Department Of Microbioanalytics
, Zbigniew Brzózka (FC / IBC / DM)
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesMicrochimica Acta, ISSN 0026-3672, [1436-5073]
Issue year2013
Vol180
No9
Pages895-901
Publication size in sheets0.5
Keywords in Englishchemotherapy resistance, multidrug tests, microfluidic gradient system, lab-on-a-chip, celecoxib, 5-fluorouracil
ASJC Classification1602 Analytical Chemistry
DOIDOI:10.1007/s00604-013-1009-4
URL http://link.springer.com/article/10.1007%2Fs00604-013-1009-4
Languageen angielski
File
microchimicaacta_2013_180_895.pdf 333.58 KB
Score (nominal)40
Score sourcejournalList
ScoreMinisterial score = 30.0, 08-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 08-01-2020, ArticleFromJournal
Publication indicators WoS Citations = 17; Scopus Citations = 18; GS Citations = 23.0; Scopus SNIP (Source Normalised Impact per Paper): 2014 = 1.035; WoS Impact Factor: 2013 = 3.719 (2) - 2013=3.118 (5)
Citation count*23 (2020-09-02)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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