A microfluidic system to study the cytotoxic effect of drugs: the combined effect of celecoxib and 5-fluorouracil on normal and cancer cells
Elżbieta Jastrzębska , Sylwia Flis , Aleksandra Rakowska , Michał Chudy , Zenon Jastrzębski , Artur Dybko , Zbigniew Brzózka
AbstractWe have investigated the response of normal and cancer cells to exposure a combination of celecoxib (Celbx) and 5-fluorouracil (5-FU) using a lab-on-a-chip microfluidic device. Specifically, we have tested the cytotoxic effect of Celbx on normal mouse embryo cells (Balb/c 3T3) and human lung carcinoma cells (A549). The single drugs or their combinations were adjusted to five different concentrations using a concentration gradient generator (CGG) in a single step. The results suggest that Celbx can enhanced the anticancer activity of 5-FU by stronger inhibition of cancer cell growth. We also show that the A549 cancer cells are more sensitive to Celbx than the Balb/c 3T3 normal cells. The results obtained with the microfluidic system were compared to those obtained with a macroscale in vitro cell culture method. In our opinion, the microfluidic system represents a unique approach for an evaluation of cellular response to multidrug exposure that also is more simple than respective microwell plate assays.
|Journal series||Microchimica Acta, ISSN 0026-3672, [1436-5073]|
|Publication size in sheets||0.5|
|Keywords in English||chemotherapy resistance, multidrug tests, microfluidic gradient system, lab-on-a-chip, celecoxib, 5-fluorouracil|
|Score|| = 30.0, 08-01-2020, ArticleFromJournal|
= 40.0, 08-01-2020, ArticleFromJournal
|Publication indicators||= 17; = 18; = 23.0; : 2014 = 1.035; : 2013 = 3.719 (2) - 2013=3.118 (5)|
|Citation count*||23 (2020-09-02)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.