Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide

Beata Aleksandra Butruk-Raszeja , Magdalena S. Dresler , Aleksandra Kuźmińska , Tomasz Ciach

Abstract

The paper presents method for chemical immobilization of arginine-glutamic acid-aspartic acid-valine (REDV) peptide on polyurethane surface. The peptide has been covalently bonded using silanes as a spacer molecules. The aim of this work was to investigate the proposed modification process and assess its biological effectiveness, especially in contact with blood and endothelial cells. Physicochemical properties were examined in terms of wettability, atomic composition and density of introduced functional groups and peptide molecules. Experiments with blood showed that material coating reduced number of surface-adhered platelets and fibrinogen molecules. In contrast to polyurethane (PU), there were no blood components deposited on REDV-modified materials (PU-REDV); fibrinogen adsorption on PU-REDV surface has been strongly reduced compared to PU. Analysis of cell adhesion after 1, 2, 3, 4, and 5 days of culture showed a significant increase of the cell-coated area on PU-REDV compared to PU. However, an intense cell growth appeared also on the control surface modified without the addition of REDV. Thus, the positive effect of REDV peptide on the adhesion of HUVEC could not be unequivocally confirmed.
Author Beata Aleksandra Butruk-Raszeja (FCPE / DBBE)
Beata Aleksandra Butruk-Raszeja,,
- Department of Biotechnology and Bioprocess Engineering
, Magdalena S. Dresler (FCPE) - [Laboratory of Biomedical Engineering, Faculty of Chemical and Process Engineering (BioMedLab) [Warsaw University of Technology (PW)]]
Magdalena S. Dresler,,
- Faculty of Chemical and Process Engineering
- Laboratorium Inżynierii Biomedycznej, Wydział Inżynierii Chemicznej i Procesowej
, Aleksandra Kuźmińska (FCPE / DBBE)
Aleksandra Kuźmińska,,
- Department of Biotechnology and Bioprocess Engineering
, Tomasz Ciach (FCPE / DBBE)
Tomasz Ciach,,
- Department of Biotechnology and Bioprocess Engineering
Journal seriesColloids and Surfaces B-Biointerfaces, ISSN 0927-7765, (A 35 pkt)
Issue year2016
Vol144
Pages335-343
Publication size in sheets0.5
Keywords in EnglishREDV, endothelial cells, polyurethane, blood compatibility, platelet adhesion, surface endothelialization
ASJC Classification1505 Colloid and Surface Chemistry; 1606 Physical and Theoretical Chemistry; 3110 Surfaces and Interfaces; 2700 General Medicine; 1305 Biotechnology
DOIDOI:10.1016/j.colsurfb.2016.04.017
URL http://www.sciencedirect.com/science/article/pii/S0927776516302740
Languageen angielski
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Butruk-Raszeja B. A. (i in.) - Endothelialization of polyurethanes....pdf 3.26 MB
Score (nominal)35
ScoreMinisterial score = 35.0, 05-09-2019, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 05-09-2019, ArticleFromJournal
Publication indicators Scopus Citations = 9; WoS Citations = 9; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.322; WoS Impact Factor: 2016 = 3.887 (2) - 2016=4.295 (5)
Citation count*14 (2020-01-23)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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