Single-shot isotropic quantitative phase microscopy based on color-multiplexed differential phase contrast

Yao Fan , Jiasong Sun , Qian Chen , Xiangpeng Pan , Maciej Trusiak , Chao Zuo

Abstract

We present a single-shot isotropic quantitative phase imaging method based on color-multiplexed differential phase contrast. In our method, the illumination source is modulated by an LCD into an annular color-multiplexed pattern matching the numerical aperture of the objective precisely to maximize the frequency response for both low and high frequencies (from 0 to 2NAobjλ). In addition, we propose an alternating illumination scheme to provide a perfectly circularly symmetrical phase transfer function, achieving isotropic imaging resolution and signal-to-noise ratio. A color camera records the light transmitted through the specimen, and three monochromatic intensity images at each color channel are then separated and utilized to recover the phase of the specimen. We display the derivation, implementation, simulation and experimental results, which demonstrate that our method accomplishes high resolution, noise-robustness and reconstruction accuracy phase reconstruction at camera-limited frame rates.
Author Yao Fan - School of Electronic and Optical Engineering [Nanjing University of Science and Technology]
Yao Fan,,
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, Jiasong Sun - School of Electronic and Optical Engineering [Nanjing University of Science and Technology]
Jiasong Sun,,
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, Qian Chen - School of Electronic and Optical Engineering [Nanjing University of Science and Technology]
Qian Chen,,
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, Xiangpeng Pan - School of Electronic and Optical Engineering [Nanjing University of Science and Technology]
Xiangpeng Pan,,
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, Maciej Trusiak (FM / IMPh)
Maciej Trusiak,,
- The Institute of Micromechanics and Photonics
, Chao Zuo - School of Electronic and Optical Engineering [Nanjing University of Science and Technology]
Chao Zuo,,
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Journal seriesAPL Photonics, ISSN 2378-0967
Issue year2020
Vol4
No12
Pages1-7
Publication size in sheets0.5
Article number121301
Keywords in Englishphase contrast microscopy, microscopy, computational physics, image processing, computational optical imaging
DOIDOI:10.1063/1.5124535
URL https://aip.scitation.org/doi/10.1063/1.5124535
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 07-09-2020, ArticleFromJournal
Publication indicators Scopus Citations = 2; WoS Citations = 1; WoS Impact Factor: 2018 = 4.383 (2) - 2018=4.383 (5)
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