Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria
Aneta Bartosik , Krzysztof Glabski , Anna Kulińska , Ewa Lewicka , Jolanta Godziszewska , Aleksandra Markowska , Grażyna Jagura-Burdzy
AbstractBackground Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use. Results To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation. Conclusion The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.
|Journal series||BMC Microbiology, ISSN 1471-2180|
|Publication size in sheets||0.7|
|Keywords in English||Broad-host-range Cloning vector RK2 lacZ xylE reporter Stability functions|
|Score|| = 30.0, 28-11-2017, ArticleFromJournal|
= 30.0, 28-11-2017, ArticleFromJournal
|Publication indicators||: 2016 = 2.644 (2) - 2016=2.99 (5)|
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