Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria

Aneta Bartosik , Krzysztof Glabski , Anna Kulińska , Ewa Lewicka , Jolanta Godziszewska , Aleksandra Markowska , Grażyna Jagura-Burdzy

Abstract

Background Low-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use. Results To facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation. Conclusion The range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.
Author Aneta Bartosik
Aneta Bartosik,,
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, Krzysztof Glabski
Krzysztof Glabski,,
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, Anna Kulińska ZTiBSL - [Institute of Biochemistry and Biophysics, Polish Academy of Sciences]
Anna Kulińska,,
- Department Of Drug Technology And Biotechnology
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences
, Ewa Lewicka
Ewa Lewicka,,
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, Jolanta Godziszewska
Jolanta Godziszewska,,
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, Aleksandra Markowska
Aleksandra Markowska,,
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, Grażyna Jagura-Burdzy
Grażyna Jagura-Burdzy,,
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Journal seriesBMC Microbiology, ISSN 1471-2180
Issue year2016
Vol16:59
Pages1-15
Publication size in sheets0.7
Keywords in EnglishBroad-host-range Cloning vector RK2 lacZ xylE reporter Stability functions
DOIDOI:10.1186/s12866-016-0674-y
URL https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-016-0674-y
Languageen angielski
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art%3A10.1186%2Fs12866-016-0674-y.pdf 1.19 MB
Score (nominal)30
ScoreMinisterial score = 30.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 2.644 (2) - 2016=2.99 (5)
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