Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin

Maciej Pilarek , Iwona Grabowska , Maria A. Ciemerych , Katarzyna Dąbkowska , Krzysztof Szewczyk

Abstract

Adherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.
Author Maciej Pilarek (FCPE / DBBE)
Maciej Pilarek,,
- Department of Biotechnology and Bioprocess Engineering
, Iwona Grabowska - [University of Warsaw (UW)]
Iwona Grabowska,,
-
- Uniwersytet Warszawski
, Maria A. Ciemerych - [University of Warsaw (UW)]
Maria A. Ciemerych,,
-
- Uniwersytet Warszawski
, Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
, Krzysztof Szewczyk (FCPE)
Krzysztof Szewczyk,,
- Faculty of Chemical and Process Engineering
Journal seriesBiotechnology Letters, ISSN 0141-5492
Issue year2013
Vol35
No9
Pages1387-1394
Publication size in sheets0.5
Keywords in Englishanimal cell culture, flexible interfacial area, liquid/liquid culture system, multiphase bioreactors, perfluorochemical (perfluorocarbon)
ASJC Classification2700 General Medicine; 1305 Biotechnology
DOIDOI:10.1007/s10529-013-1218-2
URL http://www.ncbi.nlm.nih.gov/pubmed/23666427
Languageen angielski
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Pilarek M. (i in.) - Morphology and growth....pdf 551.64 KB
Score (nominal)20
Score sourcejournalList
ScoreMinisterial score = 20.0, 23-07-2020, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 23-07-2020, ArticleFromJournal
Publication indicators WoS Citations = 20; Scopus Citations = 19; GS Citations = 23.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 0.937; WoS Impact Factor: 2013 = 1.736 (2) - 2013=1.983 (5)
Citation count*23 (2020-09-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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