Morphology and growth of mammalian cells in a liquid/liquid culture system supported with oxygenated perfluorodecalin
Maciej Pilarek , Iwona Grabowska , Maria A. Ciemerych , Katarzyna Dąbkowska , Krzysztof Szewczyk
AbstractAdherent A431, BHK-21, and C2C12 cells were cultured on a flexible interface formed between two immiscible liquid phases: (i) hydrophobic perfluorodecalin (PFD) and (ii) aqueous culture medium (DMEM). BHK-21 cells formed multicellular aggregates characterized by irregular shapes. A431, as well as C2C12 cells, grew as tight multicellular sheets of 3-D cells. Enhanced mass transfer and facilitated access of the cells to the O2 dissolved in PFD/DMEM by approx. 250 % and thereby increased the density of BHK-21 cells. Thus the liquid/liquid system is a simple, ready-to-use, and fully scalable (independent of vessel shapes); consequently it is a method for 3-D cultures of adherent animal cells in which the growth of anchorage-dependent cells is not limited by confluence effect.
|Journal series||Biotechnology Letters, ISSN 0141-5492|
|Publication size in sheets||0.5|
|Keywords in English||animal cell culture, flexible interfacial area, liquid/liquid culture system, multiphase bioreactors, perfluorochemical (perfluorocarbon)|
|Score|| = 20.0, 23-07-2020, ArticleFromJournal|
= 20.0, 23-07-2020, ArticleFromJournal
|Publication indicators||= 20; = 19; = 23.0; : 2013 = 0.937; : 2013 = 1.736 (2) - 2013=1.983 (5)|
|Citation count*||23 (2020-09-14)|
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.