Degeneration of Fraunhofer diffraction on bacterial colonies due to their light focusing properties examined in the digital holographic microscope system

Igor Buzalewicz , Kamil Liżewski , Małgorzata Kujawińska , Halina Podbielska

Abstract

The degeneration of Fraunhofer diffraction conditions in the optical system with converging spherical wave illumination for bacteriaspecies identification based on diffraction patterns is analyzed by digital holographic methods. The obtained results have shown that the colonies of analyzed bacteria species act as biological lenses with the time-dependent light focusing properties, which are characterized and monitored by means of phase retrieval from sequentially captured digital holograms. This significantly affects the location of Fraunhofer patterns observation plane,which is continuously shifted across optical axis in time.
Author Igor Buzalewicz - [Wrocław University of Science and Technology]
Igor Buzalewicz,,
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, Kamil Liżewski (FM / IMPh)
Kamil Liżewski,,
- The Institute of Micromechanics and Photonics
, Małgorzata Kujawińska (FM / IMPh)
Małgorzata Kujawińska,,
- The Institute of Micromechanics and Photonics
, Halina Podbielska - [Wrocław University of Science and Technology]
Halina Podbielska,,
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Journal seriesOptics Express, ISSN 1094-4087
Issue year2013
Vol21
No22
Pages26493-26505
Keywords in PolishCyfrowa mikroskopia holograficzna, super- rozdzielczość, pomiar kształtu mikro-obiektów
Keywords in EnglishMedical optics and biotechnology; Optical diagnostics for medicine; Digital holography; Diffraction; Optical inspection.
ASJC Classification3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.1364/OE.21.026493
Languageen angielski
Score (nominal)45
Score sourcejournalList
ScoreMinisterial score = 45.0, 07-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 07-01-2020, ArticleFromJournal
Publication indicators Scopus Citations = 6; WoS Citations = 7; GS Citations = 6.0; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 2.196; WoS Impact Factor: 2013 = 3.525 (2) - 2013=3.533 (5)
Citation count*6 (2020-09-07)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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