Multigene assessment of biodiversity of diatom (Bacillariophyceae) assemblages form the litoral zone of the Bohai and Yellow Seas in Yantai region of northeast China with some remarks on ubiquitous taxa

Andrzej Witkowski , Ch. Li , Izabela Zgłobicka , S. Yu , M.P. Ashworth , P. Dąbek , S. Qin , Ch. Tang , M. Krzywda , M. Ruppel , E.C. Theriot , R.K. Jansen , A. Car , Tomasz Płociński , Y. Wang , J.S.M. Sabir , G. Daniszewska-Kowalczyk , A. Kierzek , N.H. Hajrah

Abstract

Diatoms are important contributors to the benthic microeukaryote flora. This manuscript lays the foundation for future metagenomic and environmental sequencing projects off coastal China by curating diatom DNA sequences from the Yantai region of the Bohai and Yellow Seas (Northeast China). These studies are based on cultures established from samples collected in different seasons from marine littoral and supralittoral zones in 2013 and 2014. Thirty-six diatom strains were cultured successfully and identification of these clones was determined by light and scanning electron microscopy(LM and SEM) and DNA sequencing of the nuclear-encoded small subunit ribosomal RNA (SSU)and chloroplast-encoded rbcL and psbC genes. The strains primarily represent raphid pennate genera, such as Amphora, Amphora (Oxyamphora), Caloneis, Diploneis, Halamphora, Navicula, Nitzschia, Parlibellus, Pleurosigma, Surirella and Tryblionella. When the DNA markers from these strains were analysed in a multi-gene phylogeny, we found that some clones-particularly within the genera Amphora, Navicula and Nitzschia-show greater than expected genetic diversity despite their very similar morphology and morphometrics. We also compared the molecular and morphological identities of several seemingly ubiquitous marine littoral taxa in the genera Amphora and Nitzschia from the Indian Ocean and Atlantic Ocean, the Red Sea and Adriatic Sea to their Yellow Sea counterparts. © 2016 Coastal Education and Research Foundation.
Author Andrzej Witkowski
Andrzej Witkowski,,
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, Ch. Li
Ch. Li,,
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, Izabela Zgłobicka ZPM
Izabela Zgłobicka,,
- Division of Materials Design
, S. Yu
S. Yu,,
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, M.P. Ashworth
M.P. Ashworth,,
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, P. Dąbek
P. Dąbek,,
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, S. Qin
S. Qin,,
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, Ch. Tang
Ch. Tang,,
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, M. Krzywda
M. Krzywda,,
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, M. Ruppel
M. Ruppel,,
-
et al.
Journal seriesJournal of Coastal Research, ISSN 0749-0208
Issue year2016
Vol74
Pages166-195
Publication size in sheets1.45
Keywords in PolishOkrzemki (Bacillariophyta), Morze Bohai, Morze Żółte, strefa litoralu, metagenomika, podejście wielogenowe (rbcL, PSBC, SSU), różnorodność biologicza, biogeografia, wszechobecne taksony, Morze Czerwone, Morze Adriatyckie
Keywords in EnglishDiatoms ( Bacillariophyta ), Bohai Sea, Yellow Sea, littoral zone, metagenomics, multigene approach (rbcL, psbC, SSU), biodiversity, biogeography, ubiquistic taxa, Red Sea, Adriatic Sea
DOIDOI:10.2112/SI74-016.1
URL https://www.scopus.com/inward/record.uri?eid=2-s2.0-84975087355&partnerID=40&md5=04f32758562951f6a0e1992e910fb3bf
Languageen angielski
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Witkowski et al. 2016 JCR.pdf (file archived - login or check accessibility on faculty) Witkowski et al. 2016 JCR.pdf 1.72 MB
Score (nominal)15
ScoreMinisterial score = 15.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 15.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 0.915 (2) - 2016=1.111 (5)
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