Immunosensor Based on Long-Period Fiber Gratings for Detection of Viruses Causing Gastroenteritis

Marta Janczuk-Richter , Beata Gromadzka , Łukasz Richter , Mirosława Panasiuk , Karolina Zimmer , Predrag Mikulic , Wojtek J. Bock , Sebastian Maćkowski , Mateusz Jakub Śmietana , Joanna Niedziolka-Jonsson

Abstract

Since the norovirus is the main cause of acute gastroenteritis all over the world, its fast detection is crucial in medical diagnostics. In this work, a rapid, sensitive, and selective optical fiber biosensor for the detection of norovirus virus-like particles (VLPs) is reported. The sensor is based on highly sensitive long-period fiber gratings (LPFGs) coated with antibodies against the main coat protein of the norovirus. Several modification methods were verified to obtain reliable immobilization of protein receptors on the LPFG surface. We were able to detect 1 ng/mL norovirus VLPs in a 40-min assay in a label-free manner. Thanks to the application of an optical fiber as the sensor, there is a possibility to increase the user’s safety by separating the measurement point from the signal processing setup. Moreover, our sensor is small and light, and the proposed assay is straightforward. The designed LPFG-based biosensor could be applied in both fast norovirus detection and in vaccine testing.
Author Marta Janczuk-Richter - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Polish Academy of Sciences (PAN)]
Marta Janczuk-Richter,,
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, Beata Gromadzka
Beata Gromadzka,,
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, Łukasz Richter
Łukasz Richter,,
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, Mirosława Panasiuk
Mirosława Panasiuk,,
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, Karolina Zimmer
Karolina Zimmer,,
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, Predrag Mikulic
Predrag Mikulic,,
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, Wojtek J. Bock
Wojtek J. Bock,,
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, Sebastian Maćkowski
Sebastian Maćkowski,,
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, Mateusz Jakub Śmietana (FEIT / MO)
Mateusz Jakub Śmietana,,
- The Institute of Microelectronics and Optoelectronics
, Joanna Niedziolka-Jonsson - Institute of Physical Chemistry Polish Academy of Sciences (IChF PAN) [Polish Academy of Sciences (PAN)]
Joanna Niedziolka-Jonsson,,
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Journal seriesSensors, [SENSORS-BASEL], ISSN 1424-8220, e-ISSN 1424-3210
Issue year2020
Vol20
No3
Pages1-11
Article number813
ASJC Classification1303 Biochemistry; 1602 Analytical Chemistry; 2208 Electrical and Electronic Engineering; 3107 Atomic and Molecular Physics, and Optics
DOIDOI:10.3390/s20030813
URL https://www.mdpi.com/1424-8220/20/3/813
Projectx. Project leader: Śmietana Mateusz Jakub, , Phone: (22) 234 63 64, start date 20-05-2015, planned end date 19-05-2019, IMiO/2015/NCN/2, Implemented
WEiTI Projects financed by NSC [Projekty finansowane przez NCN]
DIAMSEC-ultra-sensitive sensory platform for rapid detection of epidemiological and pandemic threats. Project leader: Śmietana Mateusz Jakub, , Phone: (22) 234 63 64, application date 04-11-2016, start date 01-06-2017, planned end date 31-05-2020, MO/2016/7, Implemented
WEiTI Projects financed by NCRD [Projekty finansowane przez NCBiR (NCBR)]
Languageen angielski
Score (nominal)100
Score sourcejournalList
ScoreMinisterial score = 100.0, 22-06-2020, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2016 = 1.393; WoS Impact Factor: 2018 = 3.031 (2) - 2018=3.302 (5)
Citation count*1 (2020-09-22)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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