Biological behavior of titanium processed by severe plastic deformation

Dorota Kubacka , A. Yamamoto , Piotr Wieciński , Halina Garbacz

Abstract

Hydrostatic Extrusion (HE) was successfully applied to achieve grain refinement in titanium Grade 2. Cellular response (WST-1 assay) and protein adsorption tests (colloidal gold method) were performed to investigate the effects of severe plastic deformation on the biocompatibility of titanium. STEM observations were performed in order to characterize the microstructure of the deformed metal followed by correlation with it properties. This also made it possible to better understand the phenomena occurring at the surface. The surface characteristics, including wettability, surface energy and X-ray photoelectron spectroscopy (XPS), were used to determine those factors responsible for the increase in the biocompatibility of the extruded samples. The nanostructure had beneficial effect on proliferation and attachment of SaOS-2 cells. Grain refinement affected protein adsorption behavior. Non-specific adsorption represented by albumin was favored as a result of the high density of non-equilibrium defects. After the HE process, the biocompatibility of the titanium increased due to microstructural changes affecting the properties of the native passive oxide layer. Grain refinement promoted the formation of charged sites on the surface which improved protein adsorption and increased the amount of hydroxyl (OH−) groups. Surface free energy measurements revealed that this was attributed to a high acid-base component, γAB. Indirect XPS studies demonstrated that, after the HE process, the oxide layer on the titanium surface was thinner, which contributed to greater homogeneity and better corrosion resistance. The most possible explanations for the improved biocompatibility of the nanostructured materials are also discussed in this paper.
Author Dorota Kubacka (FMSE / DMD)
Dorota Kubacka,,
- Division of Materials Design
, A. Yamamoto - [National Institute for Materials Science Tsukuba]
A. Yamamoto,,
-
-
, Piotr Wieciński - [Warsaw University of Technology (PW), MNiSW [80]]
Piotr Wieciński,,
-
- Politechnika Warszawska
, Halina Garbacz (FMSE / DMD)
Halina Garbacz,,
- Division of Materials Design
Journal seriesApplied Surface Science, ISSN 0169-4332, (N/A 140 pkt)
Issue year2019
Vol472
Pages54-63
Publication size in sheets0.5
Keywords in PolishDuże odkształcenie plastyczne, Nanostrukturalny tytan, odpowiedź komórkowa, Biokompatybilność, Energia powierzchniowa, XPS
Keywords in EnglishSevere plastic deformation (SPD), Nanostructured titanium, Cell response, Biocompatibility, Surface energy, XPS
ASJC Classification2508 Surfaces, Coatings and Films
DOIDOI:10.1016/j.apsusc.2018.04.120
URL https://www.sciencedirect.com/science/article/pii/S0169433218310808
Languageen angielski
File
DKubacka.pdf 2.88 MB
Score (nominal)140
Score sourcejournalList
ScoreMinisterial score = 140.0, 06-12-2019, ArticleFromJournal
Publication indicators Scopus Citations = 1; WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2018 = 1.326; WoS Impact Factor: 2018 = 5.155 (2) - 2018=4.281 (5)
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