Hydrolyzed corn stover as a promising feedstock for 2-phenylethanol production by nonconventional yeast
Jolanta Mierzejewska , Katarzyna Dąbkowska , Karolina Chreptowicz , Aleksandra Sokołowska
AbstractBACKGROUND:Plantbiomassrichinlignocellulosesmaybeapplicablefortheproductionofhighlyvaluablebioproducts.Inthisstudy, the use of hydrolyzed corn stover as a carbon source for yeast producing 2-phenylethanol (2-PE) is investigated. 2-PE isan alcohol with a rosy scent, commonly used in the food and cosmetic industries as an aroma and preservative. The growingdemand for this compound motivates the development of new methods of production.RESULTS: The corn stover biomass was first pretreated with an alkaline solution of H2O2and secondly hydrolysed with acellulolytic enzyme cocktail to cause the saccharification of polycarbohydrates and the release of fermentable monosugars.This resulted in saccharification yields of up to 88.1±7.6% and 92.5±9.1% related to cellulose (glucan) and hemicellulose(arabinoxylan) content, respectively. Next, the obtained hydrolyzates were used as a feedstock for 2-PE production by twononconventional yeasts,Metschnikowia chrysoperlaeWUT25 andPichia fermentansWUT36. The highest 2-PE titer of 3.67 g L−1was attained with the WUT36 strain. Finally, 2-PE was extracted from the fermentation broth with ethyl acetate (100% recovery)and purified by distillation to 97.27±0.08%.CONCLUSION: This is the first report demonstrating the promising and complete route of 2-PE production by yeast in a mediumbased on the hydrolyzate of corn stover.
|Journal series||Journal of Chemical Technology and Biotechnology, ISSN 0268-2575, (N/A 70 pkt)|
|Publication size in sheets||0.5|
|Keywords in English||corn, stover, lignocellulose, hydrolyzate, 2‐phenylethanol, yeast|
|ASJC Classification||; ; ; ; ; ; ;|
|Score||= 70.0, 20-02-2020, ArticleFromJournal|
|Publication indicators||= 1; = 2; : 2018 = 0.883; : 2018 = 2.659 (2) - 2018=2.891 (5)|
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