Hydrolyzed corn stover as a promising feedstock for 2-phenylethanol production by nonconventional yeast

Jolanta Mierzejewska , Katarzyna Dąbkowska , Karolina Chreptowicz , Aleksandra Sokołowska

Abstract

BACKGROUND:Plantbiomassrichinlignocellulosesmaybeapplicablefortheproductionofhighlyvaluablebioproducts.Inthisstudy, the use of hydrolyzed corn stover as a carbon source for yeast producing 2-phenylethanol (2-PE) is investigated. 2-PE isan alcohol with a rosy scent, commonly used in the food and cosmetic industries as an aroma and preservative. The growingdemand for this compound motivates the development of new methods of production.RESULTS: The corn stover biomass was first pretreated with an alkaline solution of H2O2and secondly hydrolysed with acellulolytic enzyme cocktail to cause the saccharification of polycarbohydrates and the release of fermentable monosugars.This resulted in saccharification yields of up to 88.1±7.6% and 92.5±9.1% related to cellulose (glucan) and hemicellulose(arabinoxylan) content, respectively. Next, the obtained hydrolyzates were used as a feedstock for 2-PE production by twononconventional yeasts,Metschnikowia chrysoperlaeWUT25 andPichia fermentansWUT36. The highest 2-PE titer of 3.67 g L−1was attained with the WUT36 strain. Finally, 2-PE was extracted from the fermentation broth with ethyl acetate (100% recovery)and purified by distillation to 97.27±0.08%.CONCLUSION: This is the first report demonstrating the promising and complete route of 2-PE production by yeast in a mediumbased on the hydrolyzate of corn stover.
Author Jolanta Mierzejewska (FC / IBC / DDTB)
Jolanta Mierzejewska,,
- Department Of Drug Technology And Biotechnology
, Katarzyna Dąbkowska (FCPE / DBBE)
Katarzyna Dąbkowska,,
- Department of Biotechnology and Bioprocess Engineering
, Karolina Chreptowicz (FC / CDSB)
Karolina Chreptowicz,,
- Chair of Drug and Cosmetics Biotechnology
, Aleksandra Sokołowska (FC / CDSB)
Aleksandra Sokołowska,,
- Chair of Drug and Cosmetics Biotechnology
Journal seriesJournal of Chemical Technology and Biotechnology, ISSN 0268-2575, (A 30 pkt)
Issue year2019
Vol94
Pages777-784
Publication size in sheets0.5
Keywords in Englishcorn, stover, lignocellulose, hydrolyzate, 2‐phenylethanol, yeast
ASJC Classification1604 Inorganic Chemistry; 1605 Organic Chemistry; 2310 Pollution; 2311 Waste Management and Disposal; 2103 Fuel Technology; 2105 Renewable Energy, Sustainability and the Environment; 1500 General Chemical Engineering; 1305 Biotechnology
DOIDOI:10.1002/jctb.5823
URL https://onlinelibrary.wiley.com/doi/epdf/10.1002/jctb.5823
Languageen angielski
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Mierzejewska J. (i in.) - Hydrolyzed corn stover.pdf of 11-02-2019
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Score (nominal)30
ScoreMinisterial score = 30.0, 03-01-2019, ArticleFromJournal
Ministerial score (2013-2016) = 30.0, 03-01-2019, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.933; WoS Impact Factor: 2017 = 2.587 (2) - 2017=2.734 (5); WoS Citations = 0
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