Hierarchically structured emulsions for brain therapy

Ewa Dłuska , Agnieszka Markowska-Radomska , Agata Metera , Konrad Kosicki

Abstract

In this paper, we developed a doxorubicin-loaded double emulsion, with a specific pH-triggered drug release mechanism, and with functionalized drops’ surfaces as an effective drug (doxorubicin-DOX) delivery implant for brain tumor therapy. The functionalization of double emulsions with antibodies at oil-water interfaces was performed for targeting brain tumor cells - glioblastoma multiforme (GBM). The functionalization involved physical adsorption of antibodies: anti-CD15 (GBM-targeting surface proteins) on oil drops of double water-inoil-in-water emulsions. The interactions between molecules of antibodies and compounds of the oil drops were measured using isothermal titration calorimetry (ITC). Double emulsions with different structures and pH-sensitive polymer (sodium carboxymethylcellulose) in the external phase were prepared by a one-step method, in a Couette-Taylor flow contactor. The release rates of DOX from emulsions were analysed in phosphate buffers with a pH of 6.3 (acidic microenvironment of solid tumors) and a pH of 7.4 (normal cells). The release study demonstrated an expected morphology/structure, composition and pH-responsive controlled release behaviour. The ITC analysis showed that antibodies interacted with emulsion compounds, which was vital for functionalization of drops of emulsion for targeting cancer cells. This was demonstrated by changes in the heat rates reflecting molecular interactions and proved that proteins adhered to oil-drop interfaces.
Author Ewa Dłuska (FCPE / DPKT)
Ewa Dłuska,,
- Department of Process Kinetics and Thermodynamics
, Agnieszka Markowska-Radomska (FCPE / DPKT)
Agnieszka Markowska-Radomska,,
- Department of Process Kinetics and Thermodynamics
, Agata Metera (FCPE / DPKT)
Agata Metera,,
- Department of Process Kinetics and Thermodynamics
, Konrad Kosicki - Faculty of Biology [Uniwersytet Warszawski (UW)]
Konrad Kosicki,,
-
Journal seriesColloids and Surfaces A-Physicochemical and Engineering Aspects, ISSN 0927-7757, (A 30 pkt)
Issue year2019
Vol575
Pages205-211
Publication size in sheets0.5
Keywords in Englishantibody, adsorption, double emulsion, surface functionalization, pH-responsive emulsions, targeted drug delivery
ASJC Classification1505 Colloid and Surface Chemistry
DOIDOI:10.1016/j.colsurfa.2019.04.095
URL https://www.sciencedirect.com/science/article/pii/S0927775719304078?via%3Dihub#!
Languageen angielski
File
Dłuska E. (i in.) - Hierarchically structured emulsions....pdf 1.76 MB
Score (nominal)30
ScoreMinisterial score = 30.0, 22-07-2019, ArticleFromJournal
Publication indicators WoS Citations = 0; Scopus SNIP (Source Normalised Impact per Paper): 2017 = 1.016; WoS Impact Factor: 2017 = 2.829 (2) - 2017=2.852 (5)
Citation count*
Cite
Share Share

Get link to the record


* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
Back