Biological activity of surface modified nanocrystals

Adam Opalski , Ilona Grabowska-Jadach , Sławomir Oszwałdowski

Abstract

Considerable interest is observed in the usage of nanomaterials in medicine and medical diagnostics. Quantum dots powerful fluorescent probes, seem to be interesting agents for medical imaging that could be used to label cells for cellular tracing as well as for traceable drug/gene delivery. It is expected that their use can be crucial in pharmacokinetic and pharmacodynamic studies of drug candidates. However, their widespread and safe use must be preceded by examination of their toxicity. Method: A group of quantum dots (CdS, CdSe, CdTe), which contains cadmium ions in its core composition has been chosen for biological activity studies – examination the effect of surface chemistry for their toxicity (MTT test). Result: Quantum dots usually are hydrophobic due to inorganic core with chains of hydrophobic ligands (TOP/TOPO) remaining after synthesis. Exchange of the ligand for the hydrophilic one enables dispersion of QD in water, application to the living organisms and assessment of their influence using in vitro models. In this study, we report how modification of the surface of CdX quantum dots (with and without ZnS coating) affects their biological activity. Conclusion: Three different ligands (cysteine, dihydrolipoic acid-DHLA, and mercaptoundecanoic acid-MUA) and two cell lines MRC-5 and A549 (similar origin and function, different proliferation – one was normal, another was carcinoma) were used to test thoroughly biological activity of sur-face-modified CdX quantum dots.
Author Adam Opalski (FC / IBC / DM)
Adam Opalski,,
- Department Of Microbioanalytics
, Ilona Grabowska-Jadach (FC / IBC / DM)
Ilona Grabowska-Jadach,,
- Department Of Microbioanalytics
, Sławomir Oszwałdowski (FC / CAC)
Sławomir Oszwałdowski,,
- Chair Of Analytical Chemistry
Journal seriesCurrent Nanoscience, ISSN 1573-4137, (A 15 pkt)
Issue year2018
Vol14
Pages307-312
Publication size in sheets0.5
Keywords in EnglishQuantum dots, nanocrystals, CdX, cytotoxicity, cell culture, surface modification
ASJC Classification3003 Pharmaceutical Science; 2204 Biomedical Engineering; 2701 Medicine (miscellaneous); 1502 Bioengineering; 1305 Biotechnology
DOIDOI:10.2174/1573413714666180215152448
Languageen angielski
File
Current Nanoscience 14 (2018) 307-312.pdf 1.2 MB
Score (nominal)25
ScoreMinisterial score = 15.0, 23-12-2018, ArticleFromJournal
Ministerial score (2013-2016) = 25.0, 23-12-2018, ArticleFromJournal
Publication indicators Scopus SNIP (Source Normalised Impact per Paper): 2017 = 0.356; WoS Impact Factor: 2017 = 1.306 (2) - 2017=1.051 (5); WoS Citations = 0
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