Parallel control over surface charge and wettability using polyelectrolyte architecture: effect on protein adsorption and cell adhesion

Shifeng Guo , Xiaoying Zhu , Min Li , Liya Shi , June Lay Ting Ong , Dominik Jańczewski , Koon Gee Neoh

Abstract

Surface charge and wettability, the two prominent physical factors governing protein adsorption and cell adhesion, have been extensively investigated in the literature. However, a comparison between these driving forces in terms of their independent and cooperative effects in affecting adhesion is rarely explored on a systematic and quantitative level. Herein, we formulate a protocol that features two-dimensional control over both surface charge and wettability with limited cross-parameter influence. This strategy is implemented by controlling both the polyion charge density in the layer-by-layer (LbL) assembly process and the polyion side-chain chemical structures. The 2D property matrix spans surface isoelectric points ranging from 5 to 9 and water contact angles from 35 to 70°, with other interferential factors (e.g., roughness) eliminated. The interplay between these two surface variables influences protein (bovine serum albumin, lysozyme) adsorption and 3T3 fibroblast cell adhesion. For proteins, we observe the presence of thresholds for surface wettability and electrostatic driving forces necessary to affect adhesion. Beyond these thresholds, the individual effects of electrostatic forces and wettability are observed. For fibroblast, both surface charge and wettability have an effect on its adhesion. The combined effects of positive charge and hydrophilicity lead to the highest cell adhesion, whereas negative charge and hydrophobicity lead to the lowest cell adhesion. Our design strategy can potentially form the basis for studying the distinct behaviors of electrostatic force or wettability driven interfacial phenomena and serve as a reference in future studies assessing protein adsorption and cell adhesion to surfaces with known charge and wettability within the property range studied here.
Author Shifeng Guo
Shifeng Guo,,
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, Xiaoying Zhu
Xiaoying Zhu,,
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, Min Li
Min Li,,
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, Liya Shi
Liya Shi,,
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, June Lay Ting Ong
June Lay Ting Ong,,
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, Dominik Jańczewski LPT
Dominik Jańczewski,,
- Laboratory Of Technological Processes
, Koon Gee Neoh
Koon Gee Neoh,,
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Journal seriesACS Applied Materials & Interfaces, ISSN 1944-8244
Issue year2016
Vol8
No44
Pages30552-30563
Publication size in sheets0.55
Keywords in Englishlayer-by-layer assembly, polyelectrolyte multilayer, surface charge, surface wettability, protein adsorption, cell adhesion
DOIDOI:10.1021/acsami.6b09481
URL http://pubs.acs.org/doi/abs/10.1021/acsami.6b09481
Languageen angielski
File
2016_ACSApplMaterInter_Guo.pdf 673.03 KB
Score (nominal)40
ScoreMinisterial score = 40.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 40.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 7.504 (2) - 2016=7.823 (5)
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