Sulfhydrylated graphene-encapsulated iron nanoparticles directly aminated with polyethylenimine: a novel magnetic nanoplatform for bioconjugation of gamma globulins and polyclonal antibodies

Artur Kasprzak , Magdalena Popławska , Michał Bystrzejewski , Ireneusz P. Grudziński


This study presents for the first time the direct amination of graphene-encapsulated iron nanoparticles (GEINs) with polyethylenimine (PEI) via radical-type reaction. This work describes the first example of a direct addition of N-centered radical species onto the graphene layer. The pristine PEI and the PEI attached to GEINs have also been derivatized to introduce sulfhydryl functionalities. The proposed two-step protocol constitutes a novel, versatile and low cost method for the synthesis of polymer derivatives decorated with SH moieties. The derivatives of pristine polyethylenimine were analyzed by means of spectroscopic methods (NMR and IR), while the obtained carbon materials were studied by thermogravimetry, infrared spectroscopy, dynamic light scattering, and transmission electron microscopy. Finally, the concomitant part of this work focused on the bioconjugation type reactions of various biocompounds, including bovine gamma-globulins and human polyclonal antibodies of class IgG, with the as-obtained sulfhydrylated GEINs–PEI nanoplatform. The presence of immobilized molecules was confirmed by thermogravimetry, protein and fluorescence assays as well as confocal microscopy images.
Author Artur Kasprzak (FC / DOC)
Artur Kasprzak,,
- Department Of Organic Chemistry
, Magdalena Popławska (FC / DOC)
Magdalena Popławska,,
- Department Of Organic Chemistry
, Michał Bystrzejewski - [University of Warsaw (UW)]
Michał Bystrzejewski,,
- Uniwersytet Warszawski
, Ireneusz P. Grudziński - [Medical University of Warsaw]
Ireneusz P. Grudziński,,
Journal seriesJournal of Materials Chemistry B: materials for biology and medicine, ISSN 2050-750X
Issue year2016
Publication size in sheets0.7
Languageen angielski
wdpb_publikacje_pliki_plik_publikacja_2898_org.pdf 3 MB
Score (nominal)35
Score sourcejournalList
ScoreMinisterial score = 35.0, 02-02-2020, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 02-02-2020, ArticleFromJournal
Publication indicators WoS Citations = 11; Scopus Citations = 10; WoS Impact Factor: 2016 = 4.543 (2) - 2016=4.787 (5)
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