Sulfhydrylated graphene-encapsulated iron nanoparticles directly aminated with polyethylenimine: a novel magnetic nanoplatform for bioconjugation of gamma globulins and polyclonal antibodies

Magdalena Popławska , Artur Kasprzak , Michał Bystrzejewski , Ireneusz P. Grudziński

Abstract

This study presents for the first time the direct amination of graphene-encapsulated iron nanoparticles (GEINs) with polyethylenimine (PEI) via radical-type reaction. This work describes the first example of a direct addition of N-centered radical species onto the graphene layer. The pristine PEI and the PEI attached to GEINs have also been derivatized to introduce sulfhydryl functionalities. The proposed two-step protocol constitutes a novel, versatile and low cost method for the synthesis of polymer derivatives decorated with SH moieties. The derivatives of pristine polyethylenimine were analyzed by means of spectroscopic methods (NMR and IR), while the obtained carbon materials were studied by thermogravimetry, infrared spectroscopy, dynamic light scattering, and transmission electron microscopy. Finally, the concomitant part of this work focused on the bioconjugation type reactions of various biocompounds, including bovine gamma-globulins and human polyclonal antibodies of class IgG, with the as-obtained sulfhydrylated GEINs–PEI nanoplatform. The presence of immobilized molecules was confirmed by thermogravimetry, protein and fluorescence assays as well as confocal microscopy images.
Author Magdalena Popławska ZChO
Magdalena Popławska,,
- Department Of Organic Chemistry
, Artur Kasprzak ZChO
Artur Kasprzak,,
- Department Of Organic Chemistry
, Michał Bystrzejewski
Michał Bystrzejewski,,
-
, Ireneusz P. Grudziński
Ireneusz P. Grudziński,,
-
Journal seriesJournal of Materials Chemistry B: materials for biology and medicine, ISSN 2050-750X
Issue year2016
Vol4
No33
Pages5593-5607
Publication size in sheets0.7
DOIDOI:10.1039/c6tb00838k
URL http://pubs.rsc.org/en/content/articlelanding/2016/tb/c6tb00838k#!divAbstract
Languageen angielski
File
wdpb_publikacje_pliki_plik_publikacja_2898_org.pdf (file archived - login or check accessibility on faculty) wdpb_publikacje_pliki_plik_publikacja_2898_org.pdf 3 MB
Score (nominal)35
ScoreMinisterial score = 35.0, 28-11-2017, ArticleFromJournal
Ministerial score (2013-2016) = 35.0, 28-11-2017, ArticleFromJournal
Publication indicators WoS Impact Factor: 2016 = 4.543 (2) - 2016=4.787 (5)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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