Intensification of chondrocytes proliferation by microcarriers and wave-induced mixing: Reynolds number influence on CP5 cells growth
Authors:
- Kamil Wierzchowski,
- Aleksandra Kuźmińska,
- Maciej Pilarek
Abstract
The growth of CP5 chondrocytes on microcarriers beads in a disposable bioreactor under various conditions of wave-assisted agitation has been compared and discussed. Influences of Reynolds number for liquid (ReL) and the kLa coefficient on intensification of the growth rate (μ) and the specific yield of biomass (Y*X/S) have been identified. Culture system has been established in ReadyToProcess WAVETM25 with a 2-litre flexible bag-like container. Cytodex 3 beads have been identified as the most suitable microcarrier fulfilled CP5 chondrocytes requirements. Based on simplified dimensionless correlations, the values of ReL (i.e., 510–10,208), and kLa coefficient (i.e., 2.83–13.55 h–1), have been both determined for various presets of the WAVE 25 system. The highest values of μ = 0.0129 h–1 and Y*X/S = 10.9 × 108 cells gglc–1 have been noted for culture performed under wave-induced agitation characterised by ReL equalled to 5104. The results might be highly applicable in scaling-up, and intensifying microcarrier-engineered bioprocesses focused on adherent mammalian or human cells maintained in disposable bioreactors.
- Record ID
- WUT299d1011d5a948789aea7671eef1d320
- Author
- Journal series
- Chemical Engineering and Processing : Process Intensification, ISSN 0255-2701, e-ISSN 1873-3204
- Issue year
- 2021
- Vol
- 166
- Pages
- 1-9
- Article number
- 108472
- Keywords in English
- disposable (single-use) bioreactor, wave-type agitation, Re number, CP5 chondrocytes, microcarrier-supported cell propagation, bioprocess intensification
- ASJC Classification
- ; ; ; ;
- DOI
- DOI:10.1016/j.cep.2021.108472 Opening in a new tab
- URL
- https://www.sciencedirect.com/science/article/pii/S0255270121001732?via%3Dihub Opening in a new tab
- Related project
- [2015/17/B/ST8/00631] Miniaturowy hybrydowy układ bioreaktorowy z mieszaniem typu wave: badania i modelowanie wzrostu komórek zwierzęcych. . Project leader at WUT: Maciej Pilarek,
+48 22 234 62 72, start date 01-03-2016, end date 29-02-2020, finished, finally settled
- Language
- eng (en) English
- License
- File
-
- File: 1
- Intensification of chondrocytes proliferation by microcarriers and wave-induced mixing: Reynolds number influence on CP5 cells growth, File Wierzchowski K. (i in.) - Intensification of chondrocytes...pdf / 2 MB
- Wierzchowski K. (i in.) - Intensification of chondrocytes...pdf
- publication date: 31-05-2021
- Intensification of chondrocytes proliferation by microcarriers and wave-induced mixing: Reynolds number influence on CP5 cells growth, File Wierzchowski K. (i in.) - Intensification of chondrocytes...pdf / 2 MB
-
- Score (nominal)
- 140
- Score source
- journalList
- Score
- = 140.0, 09-05-2022, ArticleFromJournal
- Publication indicators
- = 1; = 2; = 4; : 2018 = 1.236; : 2020 (2 years) = 4.237 - 2020 (5 years) =4.055
- Citation count
- 5
- Additional fields
- Uwagi: This work was supported by the budget sources for the National Centre for Science, Poland, under OPUS-9 grant no. 2015/17/B/ST8/ 00631.
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- Uniform Resource Identifier
- https://repo.pw.edu.pl/info/article/WUT299d1011d5a948789aea7671eef1d320/
- URN
urn:pw-repo:WUT299d1011d5a948789aea7671eef1d320
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.