Intensification of chondrocytes proliferation by microcarriers and wave-induced mixing: Reynolds number influence on CP5 cells growth

Authors:

• Kamil Wierzchowski,
• Aleksandra Kuźmińska,
• Maciej Pilarek

Abstract

The growth of CP5 chondrocytes on microcarriers beads in a disposable bioreactor under various conditions of wave-assisted agitation has been compared and discussed. Influences of Reynolds number for liquid (ReL) and the kLa coefficient on intensification of the growth rate (μ) and the specific yield of biomass (Y*X/S) have been identified. Culture system has been established in ReadyToProcess WAVETM25 with a 2-litre flexible bag-like container. Cytodex 3 beads have been identified as the most suitable microcarrier fulfilled CP5 chondrocytes requirements. Based on simplified dimensionless correlations, the values of ReL (i.e., 510–10,208), and kLa coefficient (i.e., 2.83–13.55 h–1), have been both determined for various presets of the WAVE 25 system. The highest values of μ = 0.0129 h–1 and Y*X/S = 10.9 × 108 cells gglc–1 have been noted for culture performed under wave-induced agitation characterised by ReL equalled to 5104. The results might be highly applicable in scaling-up, and intensifying microcarrier-engineered bioprocesses focused on adherent mammalian or human cells maintained in disposable bioreactors.