Design and Synthesis of Polymer-Functionalized NIR Fluorescent Dyes Magnetic Nanoparticles for Bioimaging

Swee Kuan Yen , Dominik Jańczewski , Jeeva Lavanya Lakshmi , Surani Bin Dolmanan , Sudhiranjan Tripathy , Vincent H. B. Ho , Vimalan Vijayaragavan , Anushya Hariharan , Parasuraman Padmanabhan , Kishore K. Bhakoo , Thankiah Sudhaharan , Sohail Ahmed , Yong Zhang , Subramanian Tamil Selvan


The fluorescent probes having complete spectral separation between absorption and emission spectra (large Stokes shift) are highly useful for solar concentrators and bioimaging. In bioimaging application, NIR fluorescent dyes have a greater advantage in tissue penetration depth compared to visible-emitting organic dyes or inorganic quantum dots. Here we report the design, synthesis, and characterization of an amphiphilic polymer, poly(isobutylene-alt-maleic anhyride)-functionalized near-infrared (NIR) IR-820 dye and its conjugates with iron oxide (Fe3O4) magnetic nanoparticles (MNPs) for optical and magnetic resonance (MR) imaging. Our results demonstrate that the Stokes shift of unmodified dye can be tuned (from ∼106 to 208 nm) by the functionalization of the dye with polymer and MNPs. The fabrication of bimodal probes involves (i) the synthesis of NIR fluorescent dye (IR-820 cyanine) functionalized with ethylenediamine linker in high yield, >90%, (ii) polymer conjugation to the functionalized NIR fluorescent dye, and (iii) grafting the polymer-conjugated dyes on iron oxide MNPs. The resulting uniform, small-sized (ca. 6 nm) NIR fluorescent dye–magnetic hybrid nanoparticles (NPs) exhibit a wider emissive range (800–1000 nm) and minimal cytotoxicity. Our preliminary studies demonstrate the potential utility of these NPs in bioimaging by means of direct labeling of cancerous HeLa cells via NIR fluorescence microscopy and good negative contrast enhancement in T2-weighted MR imaging of a murine model.
Author Swee Kuan Yen - [A-Star, Institute of Materials Research and Engineering]
Swee Kuan Yen,,
, Dominik Jańczewski (FC / LTP) - [Institute of Materials Research and Engineering]
Dominik Jańczewski,,
- Laboratory Of Technological Processes
- Institute of Materials Research and Engineering
, Jeeva Lavanya Lakshmi - [A-Star, Institute of Materials Research and Engineering]
Jeeva Lavanya Lakshmi,,
, Surani Bin Dolmanan
Surani Bin Dolmanan,,
, Sudhiranjan Tripathy - [A-Star, Institute of Materials Research and Engineering]
Sudhiranjan Tripathy,,
, Vincent H. B. Ho - [Agency for Science, Technology and Research, Singapore]
Vincent H. B. Ho,,
, Vimalan Vijayaragavan - [A-Star, Singapore Bioimaging Consortium]
Vimalan Vijayaragavan,,
, Anushya Hariharan - [A-Star, Singapore Bioimaging Consortium]
Anushya Hariharan,,
, Parasuraman Padmanabhan - [A-Star, Singapore Bioimaging Consortium]
Parasuraman Padmanabhan,,
, Kishore K. Bhakoo - [A-Star, Singapore Bioimaging Consortium]
Kishore K. Bhakoo,,
et al.`
Journal seriesACS Nano, ISSN 1936-0851
Issue year2013
Publication size in sheets0.5
Keywords in Englishnear-infrared fluorescent dyes, magnetic nanoparticles, bimodal probes, optical imaging, magnetic resonance imaging
ASJC Classification3100 General Physics and Astronomy; 2200 General Engineering; 2500 General Materials Science
Internal identifier17/DJ
Languageen angielski
nn401734t.pdf 3.45 MB
Score (nominal)45
Score sourcejournalList
ScoreMinisterial score = 45.0, 31-01-2020, ArticleFromJournal
Ministerial score (2013-2016) = 45.0, 31-01-2020, ArticleFromJournal
Publication indicators Scopus Citations = 58; WoS Citations = 54; Scopus SNIP (Source Normalised Impact per Paper): 2013 = 2.717; WoS Impact Factor: 2013 = 12.033 (2) - 2013=13.774 (5)
Citation count*17 (2015-04-21)
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