Spraying of cell colloids in medical atomizers

Tomasz Robert Sosnowski , Anita Kurowska , Beata Aleksandra Butruk , Katarzyna Jabłczyńska

Abstract

Aerosols are often used as vehicles of medicines and their most important application is related to inhalation of anti-asthmatic or anti-inflammatory drugs. Aerosols are also convenient in topical applications, i.e. applied on skin or mucous membranes (e.g. of nasal or oral cavity). Recently, it has been shown that use of a therapeutic bioaerosol can be beneficial in the speeding up of healing processes of the skin. In this context, cell suspensions are considered as the material for which the suitable spraying method must be established in order to assure that hydrodynamic stress related to liquid atomization will not destroy the living cells. The current work is focused on testing of the influence of selected spraying techniques on the integrity and the survival of different types of cells in the atomized bio-colloid. Medical nebulizers and similar spraying devices have been characterized in respect of the size distribution of emitted droplets, and used to aerosolize selected (i.e. model) cell colloids: suspensions of yeast, bacteria and murine fibroblasts. Cell viability after spraying has been determined via direct microscopic observations and specific microbiological assays. The results shows that droplets generated from the commonly used medical inhalers (nebulizers) are too fine to contain whole living cells (median diameter of drops is around 5 m). Because the generation of small droplets requires high hydrodynamic stresses, viability of cells in the atomized suspension is strongly reduced even if cells are mechanically resistant and small in size. Tested nasal atomizer (spray pump) and Microsprayer devices produce larger droplets (median diameter of drops 50-80 um) which can contain integral fibroblast cells. Owing to lower shear stresses during atomization in these two devices, the aerosolized cells remain viable and capable of proliferation. The studies allow to conclude that only selected techniques can be effective in the atomization of bio-colloid for medical applications. Moreover, the atomization method must be accompanied by proper techniques of aerosol transfer to the bronchial tree which will prevent droplets from deposition in the upper airways. Technical concepts of such cell delivery are proposed. This paper is an extended version of our recent work published in Chemical Engineering Transactions (Sosnowski et al., 2013).
Author Tomasz Robert Sosnowski (FCPE / CIPE)
Tomasz Robert Sosnowski,,
- Chair of Integrated Process Engineering
, Anita Kurowska (FCPE)
Anita Kurowska,,
- Faculty of Chemical and Process Engineering
, Beata Aleksandra Butruk (FCPE / DBBE)
Beata Aleksandra Butruk,,
- Department of Biotechnology and Bioprocess Engineering
, Katarzyna Jabłczyńska (FCPE / CIPE)
Katarzyna Jabłczyńska,,
- Chair of Integrated Process Engineering
Pages371-380
Publication size in sheets0.5
Book Pierucci Sauro (eds.): Selected papers of The Eleventh International Conference on Chemical and Process Engineering, AIDIC Conference Series, vol. 11, 2013, Włochy, AIDIC Servizi S.r.l, ISBN 978-88-95608-55-6, 440 p.
DOIDOI:10.3303/ACOS1311038
URL http://www.aidic.it/acos/13/11/038.pdf
Languageen angielski
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Sosnowski T.R. (i in.) - Spraying of Cell....pdf 1.58 MB
Score (nominal)5
Score sourcejournalList
ScoreMinisterial score = 0.0, 27-04-2020, BookChapterSeriesAndMatConfByIndicator
Ministerial score (2013-2016) = 5.0, 27-04-2020, BookChapterSeriesAndMatConfByIndicator
Publication indicators WoS Citations = 9; GS Citations = 15.0
Citation count*15 (2020-09-20)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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