Iron in Alzheimer's and control hippocampi - Mössbauer, atomic absorption and ELISA studies

J. Gała̧zka-Friedman , E.R. Bauminger , Karol Szlachta , D. Koziorowski , R. Tomasiuk , A. Jaklewicz , Z.K. Wszołek , D. Dickson , K. Kaplińska , A. Friedman

Abstract

Alzheimer disease is a neurodegenerative process of unknown mechanism taking place in a part of the brain - hippocampus. Oxidative stress and the role of iron in it is one of the suggested mechanisms of cells death. In this study several methods were used to assess iron and iron binding compounds in human hippocampus tissues. Mössbauer spectroscopy was used for identification of the iron binding compound and determination of total iron concentration in 12 control and one Alzheimer disease sample of hippocampus. Mössbauer parameters obtained for all samples suggest that most of the iron is ferritin-like iron. The average concentration of iron determined by Mössbauer spectroscopy in control hippocampus was 45 ± 10 ng/mg wet tissue. The average concentration of iron in 10 Alzheimer disease samples determined by atomic absorption was 66 ± 13 ng/mg wet tissue. The concentration of H and L chains of ferritin in 20 control and 10 AD hippocampi was assessed with enzyme-linked immuno-absorbent assay. The concentration of H and L ferritin was higher in Alzheimer disease compared to control (19.36 ± 1.51 vs. 5.84 ± 0.55 ng/μg protein for H, and 1.39 ± 0.25 vs. 0.55 ± 0.10 for L). This 3-fold increase of the concentration of ferritin is accompanied by a small increase of the total iron concentration.
Author J. Gała̧zka-Friedman
J. Gała̧zka-Friedman,,
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, E.R. Bauminger
E.R. Bauminger,,
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, Karol Szlachta (FP / HPRD)
Karol Szlachta,,
- High Pressure Research Division
, D. Koziorowski
D. Koziorowski,,
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, R. Tomasiuk
R. Tomasiuk,,
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, A. Jaklewicz
A. Jaklewicz,,
-
, Z.K. Wszołek
Z.K. Wszołek,,
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, D. Dickson
D. Dickson,,
-
, K. Kaplińska
K. Kaplińska,,
-
, A. Friedman
A. Friedman,,
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Journal seriesActa Physica Polonica A, ISSN 0587-4246, (A 15 pkt)
Issue year2011
Vol119
No1
Pages81-83
Keywords in EnglishAlzheimer disease; Alzheimer's; Atomic absorption; Average concentration; G protein; In-control; Iron binding; Mossbauer; Neurodegenerative; Ssbauer spectroscopies; Total irons; Wet tissues, Absorption; Concentration (process); Molybdenum; Tissue, Disease control
URL http://www.scopus.com/inward/record.url?eid=2-s2.0-78651476988&partnerID=40&md5=cdc40e0f7f64d599cd6cdb1b2425e570
Score (nominal)15
Publication indicators WoS Impact Factor: 2011 = 0.444 (2) - 2011=0.404 (5)
Citation count*10 (2015-08-13)
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