From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor

Adam Muszyński , Maria Łebkowska , Agnieszka Tabernacka , Aleksandra Miłobędzka

Abstract

A laboratory scale sequencing batch reactor (SBR), fed with synthetic wastewater containing a mixture of organic compounds, was operated for nearly six months. Despite maintaining the same operational conditions, a deterioration of enhanced biological phosphorus removal (EBPR) occurred after 40 days of SBR operation. The Prel/Cupt ratio decreased from 0.28 to 0.06 P-mol C-mol−1, and C requirements increased from 11 to 32 mg C h−1 g−1 of mixed liquor suspended solids. A FISH analysis showed that the percentage of Accumulibacter in an overall community of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) dropped from 93% to 13%. An increase in abundance of Gammaproteobacteria (from 2.6% to 22%) and Alphaproteobacteria (from 1.8% to 10%) was observed. The number of Competibacter increased from 0.5% to nearly 9%. Clusters 1 and 2 of Defluviicoccus-related GAOs, not detected before deterioration, constituted 35% and 27% of Alphaproteobacteria, respectively. We concluded that lab-scale experiments should not be extended implicitly to full-scale EBPR systems because some bacterial groups are detected mainlyin lab-scale reactors. Well-defined, lab-scale operational conditions reduce the number of ecological niches available to bacteria.
Author Adam Muszyński (FEE / DB)
Adam Muszyński,,
- Department of Biology
, Maria Łebkowska (FEE / DB)
Maria Łebkowska,,
- Department of Biology
, Agnieszka Tabernacka (FEE / DB)
Agnieszka Tabernacka,,
- Department of Biology
, Aleksandra Miłobędzka (FEE / DB)
Aleksandra Miłobędzka,,
- Department of Biology
Journal seriesCentral European Journal of Biology, ISSN 1895-104X, [1644-3632 ]
Issue year2013
Vol8
No2
Pages130-142
Publication size in sheets0.6
Keywords in EnglishPolyphosphate accumulating organism (PAO), Glycogen accumulating organism (GAO), Microbial ecology
ASJC Classification1100 General Agricultural and Biological Sciences; 2400 General Immunology and Microbiology; 1300 General Biochemistry, Genetics and Molecular Biology; 2800 General Neuroscience
DOIDOI:10.2478/s11535-013-0116-2
URL From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor - Springer http://link.springer.com/article/10.2478%2Fs11535-013-0116-2
Languageen angielski
Score (nominal)20
Score sourcejournalList
ScoreMinisterial score = 20.0, 13-08-2020, ArticleFromJournal
Ministerial score (2013-2016) = 20.0, 13-08-2020, ArticleFromJournal
Publication indicators WoS Citations = 13; Scopus Citations = 13; GS Citations = 25.0; WoS Impact Factor: 2013 = 0.633 (2) - 2013=0.748 (5)
Citation count*25 (2020-09-12)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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