Synthesis of graphene oxide nanoparticles obtained by pyrolysis of citric acid
- Piotr Wyrębiak,
- Aleksandra Poniatowska,
- Tomasz Ciach
Carbon dots (CDs), which are the newly discovered fluorescent nanomaterials, have become popular in the last decade due to their unique optical properties, good biocompatibility,low toxicity, high stability in water, and ease of synthesis. One of the types of CDs are graphene oxide nanoparticles (GONPs). GONPs have a non-zero bandwidth as opposed to graphene. With good fluorescent properties, GONPs can have much potential use in various fields. The aim of the research was to synthesize graphene oxide from citric acid with nitrogen atoms incorporated in the structure of GO and to study its effect on the viability of cells cultured in vitro. The sources of nitrogen were urea, ammonia, ethanolamine, Tris(2-amino- 2-(hydroxymethyl)propane-1,3-diol). Homogeneous, mixed solutions were pyrolyzed in 200 °Cand various time variants: 1h, 1h 40 min, 2h 30 min. The analysis of the physicochemical properties of the graphene oxides was investigated by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering technique (particle size distribution), and fluorescence measurement. The acid-base characteristics was also examined. Because of the potential use in medicine, a commonly known short cytotoxicity test MTT was used for this purpose. The mouse fibroblasts cell line L929 was incubated at 37 °C for 24h with solutions of modified graphene oxide at a concentration of 1 mg/ml and 0.5 mg/ml. Particle size analysis showed that the obtained particles are characterized by a wide distribution of diameter, especially GO after synthesis with ammonia and urea. The highest relative fluorescence was obtained for graphene oxide with nitrogen groups derived from Tris. The viability for cells incubated with this material was over 70%, so it is supposed, that it has no negative influence on the viability of cells. On the contrary, GO obtained with the addition of urea and ammonia turned out to be toxic to cells (viability below 40%). The research shows also differences between graphene oxide-based materials with nitrogen depending on nitrogen sources and its properties are strongly dependent on the synthesis conditions and confirmed that graphene oxide can be a promising material in medical applications, for example, cancer diagnostics or anticancer therapy.
- Record ID
- Publication type
- Article number
- EYEC Monograph: 9th European Young Engineers Conference, 2021, Warszawa, Politechnika Warszawska, Wydział Inżynierii Chemicznej i Procesowej, 183 p., ISBN 978-83-936575-9-9
- Keywords in English
- citric acid, graphene oxide, carbon quantum dots, fluorescence, cytotoxicity
- eng (en) English
- Score (nominal)
- Uniform Resource Identifier
* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or PerishOpening in a new tab system.