Recent progress in the application of analytical techniques to anticancer metallodrug proteomics

A.R. Timerbaev , Katarzyna Pawlak , C. Gabbiani , L. Messori

Abstract

Interactions of therapeutic drugs containing metals with proteins are known to exert a great impact on the mode of action of these compounds, including drug metabolism, delivery, cell processing, and targeting. Modern analytical techniques applied to proteomic studies of metallodrugs may improve our understanding of accompanying biochemical processes, which is essential for the efficiency of treatment, the proper dosing of established metal-based cancerostatic agents, and the design and development of new drugs. Such methods basically rely on the application of mass spectrometry (or a few alternative detection techniques) for species identification, characterization, quantification, and measuring the binding parameters, directly or after separation of free parent drug and protein-bound drug fractions, using the principles of electrophoresis, chromatography or ultrafiltration. This review focuses on the development and recent advances in the field of “metallodrug proteomics” from the implementation of advanced analytical methodologies. Also addressed are emerging issues of metallodrug binding toward cellular protein targets and within real-world biological samples.
Author A.R. Timerbaev - [Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academу of Sciences]
A.R. Timerbaev,,
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, Katarzyna Pawlak (FC / CAC)
Katarzyna Pawlak,,
- Chair Of Analytical Chemistry
, C. Gabbiani - [Universita degli Studi di Firenze]
C. Gabbiani,,
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, L. Messori - [Universita degli Studi di Firenze]
L. Messori,,
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Journal seriesTrac-Trends in Analytical Chemistry, ISSN 0165-9936, (A 50 pkt)
Issue year2011
Vol30
No7
Pages1120-1138
Keywords in EnglishAnticancer metallodrug, Biological sample, Capillary electrophoresis, high-performance liquid chromatography, Mass spectrometry, Metal-based drug, Metallodrug proteomics, Metallomics, Metalloproteomics, Protein
ASJC Classification1607 Spectroscopy; 1602 Analytical Chemistry
DOIDOI:10.1016/j.trac.2011.03.007
URL http://www.sciencedirect.com/science/article/pii/S016599361100121X
Languageen angielski
Score (nominal)50
Score sourcejournalList
Publication indicators Scopus Citations = 36; WoS Citations = 36; Scopus SNIP (Source Normalised Impact per Paper): 2011 = 2.117; WoS Impact Factor: 2011 = 6.273 (2) - 2011=6.873 (5)
Citation count*26 (2014-12-20)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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