Evaluation of cytotoxic effect of 5-fluorouracil on human carcinoma cells in microfluidic system

Elżbieta Jastrzębska , S. Flis , K. Sofinska , Z. Jastrzebski , Michał Chudy , Artur Dybko , Zbigniew Brzózka

Abstract

In this paper, we present cytotoxicity tests performed in a microanalytical hybrid system. The microchambers of cell culture were integrated with a concentration gradient generator (CGG), which created five different concentrations of evaluated agent in a single step. 5-Fluorouracil (5-FU) is one of the most important chemotherapeutic agents for cancer treatment, therefore it was tested in the microsystem. Independent tests of the cytotoxic effect of 5-FU were performed in this microsystem on different cell lines (human lung carcinoma cells – A549 and human colon carcinoma cells – HT-29). We also present the dependence of cell viability at different times of incubation with 5-FU. We proved that 5-FU has a stronger inhibition effect on A549 than on HT-29 cells. Moreover, the results obtained in the microfluidic system were compared with that of a macroscale. It can be concluded that the conditions of cultivation can affect the level of cytotoxicity of drugs, and the microsystem is a good example that could be used in this field of research.
Author Elżbieta Jastrzębska ZMB
Elżbieta Jastrzębska,,
- Department Of Microbioanalytics
, S. Flis
S. Flis,,
-
, K. Sofinska
K. Sofinska,,
-
, Z. Jastrzebski
Z. Jastrzebski,,
-
, Michał Chudy ZMB
Michał Chudy,,
- Department Of Microbioanalytics
, Artur Dybko ZMB
Artur Dybko,,
- Department Of Microbioanalytics
, Zbigniew Brzózka ZMB
Zbigniew Brzózka,,
- Department Of Microbioanalytics
Journal seriesSensors and Actuators B - Chemical, ISSN 0925-4005
Issue year2011
Vol160
No1
Pages1544-1551
Keywords in English5-Fluorouracil, Cancer treatment, Cell culture, Cytotoxic tests, Lab-on-a-chip, Microfluidic system
DOIDOI:10.1016/j.snb.2011.08.074
URL http://www.sciencedirect.com/science/article/pii/S0925400511007970
Languageen angielski
Score (nominal)40
Publication indicators WoS Impact Factor: 2011 = 3.898 (2) - 2011=3.751 (5)
Citation count*13 (2015-05-30)
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