Comparison of bacterial adhesion and cellular proliferation on newly developed three-dimensional scaffolds manufactured by rapid prototyping technology

A. Al-Ahmad , C. Schubert , C. Carvalho , Y. Thoman , A. Wittmer , M. Metzger , E. Hellwig , Wojciech Święszkowski , M. Wiedmann-Al-Ahmad

Abstract

Scaffolds used in the field of tissue engineering should facilitate the adherence, spreading, and ingrowth of cells as well as prevent microbial adherence. For the first time, this study simultaneously deals with microbial and tissue cell adhesion to rapid prototyping-produced 3D-scaffolds. The cell growth of human osteosarcoma cells (CAL-72) over a time period of 3–11 days were examined on three scaffolds (PLGA, PLLA, PLLA-TCP) and compared to the adhesion of salivary microorganisms and representative germs of the oral flora (Porphyromonas gingivalis, Prevotella nigrescens, Candida albicans, Enterococcus faecalis, Streptococcus mutans, and Streptococcus sanguinis). Scanning electron microscopy (SEM), cell proliferation measurements, and determination of the colony forming units (CFU) were performed. The cell proliferation rates on PLLA and PLLA-TCP after 3, 7, and 11 days of cultivation were higher than on PLGA. On day 3 the proliferation rates on PLLA and PLLA-TCP, and on day 5 on PLLA-TCP, proved to be significantly higher compared to that of the control (culture plate). The strain which showed the most CFUs on all of the investigated scaffolds was P. gingivalis, followed by E. faecalis. No significant CFU differences were determined examining P. gingivalis among the biomaterials. In contrast, E. faecalis was significantly more adherent to PLGA and PLLA compared to PLLA-TCP. The lowest CFU values were seen with C. albicans and P. nigrescens. Salivary born aerobic and anaerobic microorganisms adhered significantly more to PLGA compared to PLLA-TCP. These results supported by SEM point out the high potential of PLLA-TCP in the field of tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.
Author A. Al-Ahmad - [Universität Freiburg im Breisgau]
A. Al-Ahmad,,
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, C. Schubert - [Universität Freiburg im Breisgau]
C. Schubert,,
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, C. Carvalho - [Universität Freiburg im Breisgau]
C. Carvalho,,
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, Y. Thoman - [Universität Freiburg im Breisgau]
Y. Thoman,,
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, A. Wittmer - [Universitäts Klinikum Freiburg und Medizinische Fakultät]
A. Wittmer,,
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, M. Metzger - [Universität Freiburg im Breisgau]
M. Metzger,,
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, E. Hellwig - [Universität Freiburg im Breisgau]
E. Hellwig,,
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, Wojciech Święszkowski (FMSE / DMD)
Wojciech Święszkowski,,
- Division of Materials Design
, M. Wiedmann-Al-Ahmad - [Universität Freiburg im Breisgau]
M. Wiedmann-Al-Ahmad,,
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Journal seriesJournal of Biomedical Materials Research Part A, ISSN 1552-4965, (0 pkt)
Issue year2011
Vol98A
No2
Pages303–311
Keywords in Englishbacterial colonization, cell proliferation, dental pathogens, rapid prototyping, tissue engineering
DOIDOI:10.1002/jbm.a.33118
URL http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.33118/abstract
Score (nominal)0
Score sourcejournalList
Publication indicators Scopus Citations = 10; WoS Citations = 10
Citation count*5 (2015-05-14)
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* presented citation count is obtained through Internet information analysis and it is close to the number calculated by the Publish or Perish system.
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